Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
740,531 |
T→C |
F201F (TTT→TTC) |
phr → |
deoxyribodipyrimidine photolyase, FAD‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 740,531 | 0 | T | C | 77.4%
| 114.1
/ 29.4
| 62 | F201F (TTT→TTC) | phr | deoxyribodipyrimidine photolyase, FAD‑binding |
| Reads supporting (aligned to +/- strand): ref base T (12/2); new base C (48/0); total (60/2) |
| Fisher's exact test for biased strand distribution p-value = 4.81e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 |
ATCCTCGTCAGTCTTTCGATACTGCGCATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTACGCCAGTTTTGCCAGAACGGTGC > W3110S.gb/740500‑740587
|
aTCCTCGTCAGTCTTTCGATACTGCGCATTTTCGGGTGGAAGAAAAAGCGGCGa > 1:2733968/1‑54 (MQ=255)
aTCCTCGTCAGTCTTTCGATACTGCGCATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCa > 1:2305946/1‑61 (MQ=255)
aTCCTCGTCAGTCTTTCGATACTGCGCATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCa > 1:2107174/1‑61 (MQ=255)
aTCCTCGTCAGTCTTTCGATACTGCGCATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCa > 1:2000188/1‑61 (MQ=255)
aTCCTCGTCAGTCTTTCGATACTGCGCATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCa > 1:1906841/1‑61 (MQ=255)
aTCCTCGTCAGTCTTTCGATACTGCGCATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCa > 1:2874385/1‑61 (MQ=255)
aTCCTCGTCAGTCTTTCGATACTGCGCATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCa > 1:2192729/1‑61 (MQ=255)
aTCCTCGTCAGTCTTTCGATACTGCGCATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCa > 1:57444/1‑61 (MQ=255)
aTCCTCGTCAGTCTTTCGATACTGCGCATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCa > 1:1413537/1‑61 (MQ=255)
aTCCTCGTCAGTCTTTCGATACTGCGCATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCa > 1:1397233/1‑61 (MQ=255)
aTCCTCGTCAGTCTTTCGATACTGCGCATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCa > 1:1337470/1‑61 (MQ=255)
aTCCTCGTCAGTCTTTCGATACTGCGCATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCa > 1:1112301/1‑61 (MQ=255)
tCTTTCTATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:1977880/1‑55 (MQ=39)
tCTTTCGATACTGCGCATTTCCCGTTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:53155/1‑55 (MQ=39)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAg > 1:403132/1‑37 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGAt > 1:605498/1‑44 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:2655277/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:2477407/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:2722110/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:2648094/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:2643428/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:2722648/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:2552925/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:2780278/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:2373958/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:2817205/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:2863494/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:2871385/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:298459/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:395014/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:537567/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:588118/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:621916/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:659573/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:797842/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:892384/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:1944263/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:1165596/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:1188060/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:1122766/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:1455494/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:1583914/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:1663051/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:1697059/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:1848490/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:1875898/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:1884064/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:2458769/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:1977035/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:2044028/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:2147645/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:2151146/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:2171145/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:2263061/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:1040082/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:2403788/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:2426722/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:1077629/1‑55 (MQ=255)
tCTTTCGATACTGCGCATTTCCCGGCGGAAGaaaaa > 1:276573/1‑36 (MQ=37)
tCTTTCGACACTGCGCATTTCCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTAc > 1:1906202/1‑55 (MQ=39)
cATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTACGCCAGTTTTGCCAGAACGGTGc < 1:1347110/62‑1 (MQ=255)
cATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTACGCCAGTTTTGCCAGAACGGTGc < 1:2445056/62‑1 (MQ=255)
|
ATCCTCGTCAGTCTTTCGATACTGCGCATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTACGCCAGTTTTGCCAGAACGGTGC > W3110S.gb/740500‑740587
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
