minE Evolution: EZR_Evolution
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Mutations |
Converged Mutations |
Fixed Mutations |
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Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A5 F28 I3 R1 | 375 | 42.1 | 2927644 | 71.4% | 2090337 | 57.4 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,222,851 | C→A | pseudogene (474/1422 nt) pseudogene (69/1017 nt) |
ycgH → ycgH → |
ECK1157:JW5901+JW5176:b4491; hypothetical protein ECK1157:JW5176:b1170; hypothetical protein, C‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,222,851 | 0 | C | A | 100.0% | 128.9 / NA | 39 | pseudogene (474/1422 nt) pseudogene (69/1017 nt) | ycgH ycgH | ECK1157:JW5901+JW5176:b4491; hypothetical protein ECK1157:JW5176:b1170; hypothetical protein, C‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (0/39); total (0/39) | |||||||||||
TCAACAGAAACGCCTACCCCCGTCCTGCGCCCCGAAGCTGGAAGTTACGTCGCTAACCTGGC > W3110S.gb/1222822‑1222883 | tCAACAGAAATGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:177669/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:288268/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:245853/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:2466436/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:249256/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:2497037/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:2565264/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:2600852/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:2606208/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:1168327/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:418958/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:439306/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:502314/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:571536/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:650502/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:873292/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:948664/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:2235677/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:1152234/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:132135/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:1430231/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:1440825/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:1691769/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:1772951/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:19916/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:202080/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:2172098/62‑1 (MQ=255)tCAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:2360108/62‑1 (MQ=255) cAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:2640454/61‑1 (MQ=255) cAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:2884798/61‑1 (MQ=255) cAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:1633541/61‑1 (MQ=255) cAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:2042834/61‑1 (MQ=255) cAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:1428892/61‑1 (MQ=255) cAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:647055/61‑1 (MQ=255) cAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:690918/61‑1 (MQ=255) cAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:761558/61‑1 (MQ=255) cAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:1190108/61‑1 (MQ=255) cAACAGAAACGCCTACCCCCGTCCTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:987770/61‑1 (MQ=255) ccTGCGACCCGAAGCTGGCAGTTACGTCGCCAACCTGGc < 1:654193/39‑1 (MQ=25) | TCAACAGAAACGCCTACCCCCGTCCTGCGCCCCGAAGCTGGAAGTTACGTCGCTAACCTGGC > W3110S.gb/1222822‑1222883 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |