Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,614,294 |
T→A |
P224P (CCA→CCT) |
yneH ← |
predicted glutaminase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,614,294 | 0 | T | A | 92.4%
| 204.0
/ 6.2
| 66 | P224P (CCA→CCT) | yneH | predicted glutaminase |
| Reads supporting (aligned to +/- strand): ref base T (5/0); new base A (0/61); total (5/61) |
| Fisher's exact test for biased strand distribution p-value = 1.12e-07 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 |
GCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTC > W3110S.gb/1614257‑1614318
|
gcgcGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTc > 1:1056047/1‑62 (MQ=255)
gcgcGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTc > 1:1116073/1‑62 (MQ=255)
gcgcGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTc > 1:1266851/1‑62 (MQ=255)
gcgcGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTc > 1:2772693/1‑62 (MQ=255)
gcgcGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTc > 1:494451/1‑62 (MQ=255)
tGCCGCGGCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:126302/43‑1 (MQ=38)
tGCCGCGCCTGCATTGTCGTCACCACAGGTTCATCAATATGaa < 1:2903452/43‑1 (MQ=38)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATGAATATGaa < 1:1128231/43‑1 (MQ=38)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:2561038/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:2821506/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:2799402/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:2618809/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:258142/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:2580031/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:2567934/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:2566906/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:367012/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:2446330/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:2431401/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:2402984/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:2317768/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:2893972/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:327173/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:217621/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:389862/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:395043/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:445798/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:571836/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:633931/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:681944/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:693732/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:70975/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:875868/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:87861/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:926071/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:1705365/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:1092790/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:1112614/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:1266100/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:1367409/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:1370835/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:1435160/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:1438987/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:1460312/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:1515839/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:1521079/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:1532539/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:1546034/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:1666674/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:1704455/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:2292624/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:1713152/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:1751296/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:178709/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:1836268/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:1908121/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:1937224/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:1943089/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:2023242/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:2089514/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:2135765/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:2145368/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:2154844/43‑1 (MQ=255)
tGCCGCGCCTGCATTGGCGTCACCACAGGATCATCAATATGaa < 1:1858569/43‑1 (MQ=38)
agCCGCGCCTGCATTGGCGTCACCACAGGTTCATCAATATGaa < 1:782504/42‑1 (MQ=38)
cTGCATTGGCGTCACCACAGGATCATCAATATGaa < 1:2743028/35‑1 (MQ=37)
|
GCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTC > W3110S.gb/1614257‑1614318
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
