Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,089,766 |
T→G |
A210A (GCA→GCC) |
yeeY ← |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,089,766 | 0 | T | G | 86.0%
| 171.0
/ 12.0
| 57 | A210A (GCA→GCC) | yeeY | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (0/8); new base G (49/0); total (49/8) |
| Fisher's exact test for biased strand distribution p-value = 6.05e-10 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTCCAGTTTGGTTTTAAAATCGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGATGCCGTAGAAGCGCCTGCCTG > W3110S.gb/2089706‑2089786
|
cTCCAGTTTGGTTTTAAAATCGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGATg < 1:2640980/62‑1 (MQ=255)
cTCCAGTTTGGTTTTAAAATCGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGATg < 1:2139062/62‑1 (MQ=255)
cTCCAGTTTGGTTTTAAAATCGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGATg < 1:2218824/62‑1 (MQ=255)
cTCCAGTTTGGTTTTAAAATCGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGATg < 1:2768443/62‑1 (MQ=255)
cTCCAGTTTGGTTTTAAAATCGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGATg < 1:2259561/62‑1 (MQ=255)
cTCCAGTTTGGTTTTAAAATCGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGATg < 1:840567/62‑1 (MQ=255)
tCCAGTTTGGTTTTAAAATCGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGATg < 1:1450911/61‑1 (MQ=255)
ttttAAAATCGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGATg < 1:2800337/51‑1 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:556969/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:2776617/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:2809868/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:2704194/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:333218/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:476088/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:481383/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:2649712/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:499652/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:524361/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:254545/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:560526/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:589985/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:633548/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:646955/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:649168/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:718024/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:76298/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:840916/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:84105/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:95209/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:982210/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:989409/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:2601937/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:1159976/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:1217331/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:1267061/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:127667/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:1291263/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:1419514/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:1460540/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:1472837/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:1518011/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:1534349/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:1681664/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:1708091/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:1802364/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:1863816/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:1966874/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:216421/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:2174563/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:2209620/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:2248306/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:2359009/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:2360621/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:2542431/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:1055692/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGAGGCCGTAGAAGCGcctgcctg > 1:2607106/1‑61 (MQ=255)
cGAAAACGGTAATGGCTTCTTGTTCGACGAGAAGTTGTGAGGCCGTAGAAGCgcctgc > 1:1148631/1‑58 (MQ=255)
|
CTCCAGTTTGGTTTTAAAATCGAAAACGGTAATGGCTTCTTGTTCGTCGAGAAGTTGTGATGCCGTAGAAGCGCCTGCCTG > W3110S.gb/2089706‑2089786
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
