Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,444,631 |
G→A |
T312T (ACC→ACT) |
yfcJ ← |
predicted transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,444,631 | 0 | G | A | 100.0%
| 59.7
/ NA
| 19 | T312T (ACC→ACT) | yfcJ | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/19); total (0/19) |
GAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCA > W3110S.gb/2444617‑2444678
|
gAACATCCGGCTCCAGTTAACGCCGCGCCCGCTAATGCGACTCACGCCCCTGGTGCTTGCCa < 1:1033373/62‑1 (MQ=255)
gAACATCCGGCTCCAGTTAACGCCGCGCCCGCTAATGCGACCCACGCCCCTGGTGCTTGCCa < 1:2125713/62‑1 (MQ=255)
gAACATCCGGCTCCAGTTAACGCCGCGCCCGCTAATGCGACCCACGCCCCTGGTGCTTGCCa < 1:968151/62‑1 (MQ=255)
gAACATCCGGCTCCAGTTAACGCCGCGCCCGCTAATGCGACCCACGCCCCTGGTGCTTGCCa < 1:745311/62‑1 (MQ=255)
gAACATCCGGCTCCAGTTAACGCCGCGCCCGCTAATGCGACCCACGCCCCTGGTGCTTGCCa < 1:741317/62‑1 (MQ=255)
gAACATCCGGCTCCAGTTAACGCCGCGCCCGCTAATGCGACCCACGCCCCTGGTGCTTGCCa < 1:610522/62‑1 (MQ=255)
gAACATCCGGCTCCAGTTAACGCCGCGCCCGCTAATGCGACCCACGCCCCTGGTGCTTGCCa < 1:406184/62‑1 (MQ=255)
gAACATCCGGCTCCAGTTAACGCCGCGCCCGCTAATGCGACCCACGCCCCTGGTGCTTGCCa < 1:40548/62‑1 (MQ=255)
gAACATCCGGCTCCAGTTAACGCCGCGCCCGCTAATGCGACCCACGCCCCTGGTGCTTGCCa < 1:2848112/62‑1 (MQ=255)
gAACATCCGGCTCCAGTTAACGCCGCGCCCGCTAATGCGACCCACGCCCCTGGTGCTTGCCa < 1:2614717/62‑1 (MQ=255)
gAACATCCGGCTCCAGTTAACGCCGCGCCCGCTAATGCGACCCACGCCCCTGGTGCTTGCCa < 1:2056011/62‑1 (MQ=255)
gAACATCCGGCTCCAGTTAACGCCGCGCCCGCTAATGCGACCCACGCCCCTGGTGCTTGCCa < 1:1350969/62‑1 (MQ=255)
gAACATCCGGCTCCAGTTAACGCCGCGCCCGCTAATGCGACCCACGCCCCTGGTGCTTGCCa < 1:1293550/62‑1 (MQ=255)
gAACATCCGGCTCCAGTTAACGCCGCGCCCGCTAATGCGACCCACGCCCCTGGTGCTTGCCa < 1:123455/62‑1 (MQ=255)
gAACATCCGGCTCCAGTTAACGCCGCGCCCGCTAATGCGACCCACGCCCCTGGTGCTTGCCa < 1:1064495/62‑1 (MQ=255)
gAACATCCGGCTCCAGTTAACGCCGCGCCCGCTAATGCGACCCACGCCCCTGGTGCTTGCCa < 1:103673/62‑1 (MQ=255)
gAACATCCGGCTCCAGTTAACGCCGCGCCCGCTAATGAGACCCACGCCCCTGGTGCTTGCCa < 1:2629080/62‑1 (MQ=255)
aaCATCCGGCTCCAGTTAACGCCGCGCCCGCTAATGCGACCCACGCCCCTGGTGCTTGCCa < 1:1660731/61‑1 (MQ=255)
aaCATCCGGCTCCAGTTAACGCCGCGCCCGCTAATGCGACCCACGCCCCTGGTGCTTGCCa < 1:1147227/61‑1 (MQ=255)
|
GAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCA > W3110S.gb/2444617‑2444678
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
