Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,446,218 |
T→C |
V278V (GTA→GTG) |
fabB ← |
3‑oxoacyl‑[acyl‑carrier‑protein] synthase I |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,446,218 | 0 | T | C | 75.6%
| 142.2
/ 44.6
| 82 | V278V (GTA→GTG) | fabB | 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I |
| Reads supporting (aligned to +/- strand): ref base T (16/4); new base C (0/62); total (16/66) |
| Fisher's exact test for biased strand distribution p-value = 1.16e-13 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTA > W3110S.gb/2446166‑2446274
|
tCAGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:2076361/61‑1 (MQ=255)
aGGTAGTTGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1885131/59‑1 (MQ=25)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:2833514/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:2858507/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:2767995/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:2734142/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:2731178/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:2635500/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:2610200/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:2580653/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:2538987/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:2490720/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:2445414/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:2382702/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:2251937/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:215429/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:2151125/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:589520/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:971533/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:925886/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:851256/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:805576/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:745185/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:699267/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:698497/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:616688/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1074608/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:567049/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:554661/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:466006/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:427274/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:339572/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:306714/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:2908377/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1683245/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1007064/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1075597/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1116398/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1181380/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1247001/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1270220/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1377431/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1455447/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1460946/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:15551/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1623634/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1624736/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1657387/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:2106197/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1721650/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:2055063/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:2049602/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:2005785/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:199924/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1896963/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:198056/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1903644/59‑1 (MQ=255)
aGGTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1929644/59‑1 (MQ=255)
aGGTAGTCGATT‑GGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:2110416/58‑1 (MQ=255)
ggTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1811611/58‑1 (MQ=255)
ggTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1620054/58‑1 (MQ=255)
ggTAGTCGATTGGGGTATCAACACCGTGCATCGCCATCTTCATGCAGCGCACTGCAcc < 1:1591268/58‑1 (MQ=255)
aTCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTTCGCCTTCGCCAg < 1:264628/62‑1 (MQ=255)
aTCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAg < 1:155057/62‑1 (MQ=255)
aTCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAg < 1:2183619/62‑1 (MQ=255)
aTCGATTGGGGTATCAACGCCATGCATCGCCATATTCATGCAGCGTACTGCGCCTTCGCCAg < 1:537471/62‑1 (MQ=255)
tCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGa > 1:2147744/1‑62 (MQ=255)
tCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTgcg > 1:961946/1‑62 (MQ=255)
tCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTgcg > 1:2714105/1‑62 (MQ=255)
tCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTgcg > 1:2522274/1‑62 (MQ=255)
tCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTgcg > 1:2665579/1‑62 (MQ=255)
tCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTgcg > 1:612886/1‑62 (MQ=255)
tCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTgcg > 1:2241101/1‑62 (MQ=255)
tCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTgcg > 1:694182/1‑62 (MQ=255)
tCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTgcg > 1:2920415/1‑62 (MQ=255)
tCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTgcg > 1:1967392/1‑62 (MQ=255)
tCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTgc > 1:2668882/1‑61 (MQ=255)
tCATGCAGCGTACTGCGCCTTAGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTgcg > 1:2198970/1‑62 (MQ=255)
aGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTa > 1:2441177/1‑61 (MQ=255)
aGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTa > 1:1348102/1‑61 (MQ=255)
aGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTa > 1:1602771/1‑61 (MQ=255)
aGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTa > 1:1861037/1‑61 (MQ=255)
|
TCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTA > W3110S.gb/2446166‑2446274
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
