Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,053,894 |
A→G |
R69R (CGT→CGC) |
ygfB ← |
hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,053,894 | 0 | A | G | 100.0%
| 241.1
/ NA
| 70 | R69R (CGT→CGC) | ygfB | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (0/70); total (0/70) |
AAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGT > W3110S.gb/3053845‑3053906
|
ataGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2616570/60‑1 (MQ=255)
aaaGGAAGCCGTCATCCTTCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2287386/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGTCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2479936/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2556017/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2744605/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2737153/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2730949/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:272311/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2701259/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2643002/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2571808/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2770096/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2511454/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2458141/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1041310/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2403403/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2386761/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2454685/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:27821/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2881359/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2899013/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:334455/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:355712/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:400086/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:429543/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:64768/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:699953/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:70842/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:725287/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:905758/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:956153/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:967535/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:975665/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:148329/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1046744/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1051642/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1106676/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1108466/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1153130/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1174654/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1206145/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1240623/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1272098/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1443433/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1458654/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2368413/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1535992/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2323849/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2300887/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1731839/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1749716/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1845150/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1949593/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2205433/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1617631/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2045580/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:208634/62‑1 (MQ=255)
aaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2180603/62‑1 (MQ=255)
aaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:569384/61‑1 (MQ=255)
aaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2252887/61‑1 (MQ=255)
aaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:951693/61‑1 (MQ=255)
aaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:946337/61‑1 (MQ=255)
aaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:2514914/61‑1 (MQ=255)
aaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:47767/61‑1 (MQ=255)
aaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1435450/61‑1 (MQ=255)
aaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:172882/61‑1 (MQ=255)
aaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1536866/61‑1 (MQ=255)
aGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:935577/60‑1 (MQ=255)
gCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1611997/55‑1 (MQ=255)
cGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTGCGCAGTGCCtgt < 1:1359564/53‑1 (MQ=255)
|
AAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGT > W3110S.gb/3053845‑3053906
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
