Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,826,781 |
(T)5→6 |
coding (906/1221 nt) |
dfp ← |
fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase, FMN‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,826,776 | 1 | . | T | 100.0%
| 21.0
/ NA
| 8 | M304K (ATG→AAG) | dfp | fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase, FMN‑binding |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (8/0); total (8/0) |
TGGTCTTTTAGTGCGGCAACGCCTGCGACGATATCGGGGTTTTTAACCA‑TTTTTATTGTTA > W3110S.gb/3826728‑3826788
|
tGGTCTTTTAGTGCGGCAACGCCTGCGACGATATCGGGGTTTTTAACCATTTTTTATTGTTa > 1:1100035/1‑62 (MQ=255)
tGGTCTTTTAGTGCGGCAACGCCTGCGACGATATCGGGGTTTTTAACCATTTTTTATTGTTa > 1:1693653/1‑62 (MQ=255)
tGGTCTTTTAGTGCGGCAACGCCTGCGACGATATCGGGGTTTTTAACCATTTTTTATTGTTa > 1:1921067/1‑62 (MQ=255)
tGGTCTTTTAGTGCGGCAACGCCTGCGACGATATCGGGGTTTTTAACCATTTTTTATTGTTa > 1:2591509/1‑62 (MQ=255)
tGGTCTTTTAGTGCGGCAACGCCTGCGACGATATCGGGGTTTTTAACCATTTTTTATTGTTa > 1:2744598/1‑62 (MQ=255)
tGGTCTTTTAGTGCGGCAACGCCTGCGACGATATCGGGGTTTTTAACCATTTTTTATTGTTa > 1:2919086/1‑62 (MQ=255)
tGGTCTTTTAGTGCGGCAACGCCTGCGACGATATCGGGGTTTTTAACCATTTTTTATTGTTa > 1:930379/1‑62 (MQ=255)
tGGTCTTTTAGTGCGGCAACGCCTGCGACGATATCGGGGTTTTTAACCATTTTTTATTGTTa > 1:93671/1‑62 (MQ=255)
|
TGGTCTTTTAGTGCGGCAACGCCTGCGACGATATCGGGGTTTTTAACCA‑TTTTTATTGTTA > W3110S.gb/3826728‑3826788
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
