Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,344,845 |
G→A |
T120T (ACC→ACT) |
adiA ← |
biodegradative arginine decarboxylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,344,845 | 0 | G | A | 100.0%
| 17.1
/ NA
| 7 | T120T (ACC→ACT) | adiA | biodegradative arginine decarboxylase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (7/0); total (7/0) |
GGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCG > W3110S.gb/4344815‑4344875
|
ggCAACGGCGCGTCCGGCGATAAAGTCGGCAGTATCTTCCAGAATCCAg > 1:2133494/1‑49 (MQ=255)
ggCAACGGCGCGTCCGGCGATAAAGTCGGCAGTATCTTCCAGAATCCAGGCGAATTCa > 1:1410120/1‑58 (MQ=255)
ggCAACGGCGCGTCCGGCGATAAAGTCGGCAGTATCTTCCAGAATCCAGGCGAATTCATc > 1:2174853/1‑60 (MQ=255)
ggCAACGGCGCGTCCGGCGATAAAGTCGGCAGTATCTTCCAGAATCCAGGCGAATTCATCg > 1:1409911/1‑61 (MQ=255)
ggCAACGGCGCGTCCGGCGATAAAGTCGGCAGTATCTTCCAGAATCCAGGCGAATTCATCg > 1:1828538/1‑61 (MQ=255)
ggCAACGGCGCGTCCGGCGATAAAGTCGGCAGTATCTTCCAGAATCCAGGCGAATTCATCg > 1:2565883/1‑61 (MQ=255)
ggCAACGGCGCGTCCGGCGATAAAGTCGGCAGTATCTTCCAGAATCCAGGCGAATTCATCg > 1:476015/1‑61 (MQ=255)
|
GGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCG > W3110S.gb/4344815‑4344875
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
