Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,609,129 |
(GAT)3→4 |
coding (234/624 nt) |
bglJ → |
DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,609,120 | 1 | . | G | 100.0%
| 14.7
/ NA
| 6 | A75? (GCG→GCN) | bglJ | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (0/6); total (0/6) |
| * | W3110S.gb | 4,609,120 | 2 | . | A | 100.0%
| 14.0
/ NA
| 6 | A75A (GCG→GCA) | bglJ | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (0/6); total (0/6) |
| * | W3110S.gb | 4,609,120 | 3 | . | T | 100.0%
| 13.7
/ NA
| 6 | A75A (GCG→GCT) | bglJ | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (0/6); total (0/6) |
CGGCGTTTAGTTATTGCG‑‑‑GATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGC > W3110S.gb/4609103‑4609160
|||
cGGCGTTTAGTTATTGCGGATGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGc < 1:1247990/61‑1 (MQ=255)
cGGCGTTTAGTTATTGCGGATGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGc < 1:1371021/61‑1 (MQ=255)
cGGCGTTTAGTTATTGCGGATGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGc < 1:2026832/61‑1 (MQ=255)
cGGCGTTTAGTTATTGCGGATGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGc < 1:2667616/61‑1 (MQ=255)
cGGCGTTTAGTTATTGCGGATGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGc < 1:289824/61‑1 (MQ=255)
cGGCGTTTAGTTATTGCGGATGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGc < 1:300781/61‑1 (MQ=255)
|||
CGGCGTTTAGTTATTGCG‑‑‑GATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGC > W3110S.gb/4609103‑4609160
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
