Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I2 R1 52 300.6 3297834 97.8% 3225281 57.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,428,952 G→A R211C (CGT→TGT)  wzzE ← Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,428,9520GA100.0% 425.3 / NA 131R211C (CGT→TGT) wzzEEntobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (114/17);  total (114/17)

Only 100 of 131 total aligned reads displayed.

TCGTAGATGGCTTTCGCCACCTCTTCCTGACGCTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCATGCGC  >  minE/2428921‑2428996
                               |                                            
tCGTAGATGGCTTTCGCCACCTCTTCCTGACACTTc                                          >  1:1692248/1‑36 (MQ=255)
tCGTAGATGGCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTAcg                >  1:2537439/1‑62 (MQ=255)
tCGTAGATGGCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTAcg                >  1:2003768/1‑62 (MQ=255)
tCGTAGATGGCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTAcg                >  1:1956899/1‑62 (MQ=255)
tCGTAGATGGCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTAcg                >  1:8110/1‑62 (MQ=255)
tCGTAGATGGCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTAcg                >  1:1826631/1‑62 (MQ=255)
tCGTAGATGGCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTAcg                >  1:1812237/1‑62 (MQ=255)
tCGTAGATGGCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTAcg                >  1:1811510/1‑62 (MQ=255)
tCGTAGATGGCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTAcg                >  1:1762021/1‑62 (MQ=255)
tCGTAGATGGCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTAcg                >  1:2266724/1‑62 (MQ=255)
tCGTAGATGGCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTAcg                >  1:1640096/1‑62 (MQ=255)
tCGTAGATGGCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTAcg                >  1:3145096/1‑62 (MQ=255)
tCGTAGATGGCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTAcg                >  1:3239705/1‑62 (MQ=255)
tCGTAGATGGCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTAcg                >  1:2662829/1‑62 (MQ=255)
tCGTAGATGGCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTAcg                >  1:520741/1‑62 (MQ=255)
tCGTAGATGGCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTAcg                >  1:1144425/1‑62 (MQ=255)
tCGTAGATGGCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTAcg                >  1:2463173/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGTTTTCATCTGGATGGTACGCGCCGccc        >  1:2203663/1‑61 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCtt                                >  1:2446195/1‑37 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATTGTACGCGCCGCCCa       >  1:712688/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCgccgccaa       >  1:2752336/1‑60 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGccc        >  1:1271674/1‑61 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:596025/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:2994899/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:286153/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:2837161/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:2628439/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:278078/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:576695/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:2743054/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:2734300/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:3048628/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:419703/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:361607/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:360493/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:35670/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:436313/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:3266260/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:468014/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:3128846/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:2896138/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:3042677/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:303164/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:526780/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:677093/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:535981/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:2903566/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:2901310/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:1847101/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:1019534/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:1127164/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:1132337/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:1147382/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:1155970/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:1238544/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:1317918/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:958952/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:1457826/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:148515/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:1606079/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:1691443/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:1726015/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:1731256/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:955435/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:1922070/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:806929/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:1988447/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:2061989/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:2072267/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:2107215/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:225150/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:2264275/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:2318774/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:2352947/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:628477/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:2401890/1‑62 (MQ=255)
         gCTTTCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCa       >  1:2515565/1‑62 (MQ=255)
           tttCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTg                         >  1:599344/1‑42 (MQ=255)
           tttCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCAt      >  1:1036624/1‑61 (MQ=255)
           tttCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCAt      >  1:2545061/1‑61 (MQ=255)
           tttCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCATg     >  1:3282206/1‑62 (MQ=255)
           tttCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCATg     >  1:854550/1‑62 (MQ=255)
           tttCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCATg     >  1:193250/1‑62 (MQ=255)
           tttCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCATg     >  1:2313738/1‑62 (MQ=255)
           tttCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCATg     >  1:636395/1‑62 (MQ=255)
           tttCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCATg     >  1:2920284/1‑62 (MQ=255)
           tttCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCATg     >  1:3005305/1‑62 (MQ=255)
           tttCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCATg     >  1:503266/1‑62 (MQ=255)
           tttCGCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCATg     >  1:492776/1‑62 (MQ=255)
               gCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCGCATgcgc  <  1:3210096/61‑1 (MQ=255)
               gCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCATgcgc  <  1:2702140/61‑1 (MQ=255)
               gCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCATgcgc  <  1:2654282/61‑1 (MQ=255)
               gCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCATgcgc  <  1:2391986/61‑1 (MQ=255)
               gCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCATgcgc  <  1:2177897/61‑1 (MQ=255)
               gCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCATgcgc  <  1:1969366/61‑1 (MQ=255)
               gCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCATgcgc  <  1:3250210/61‑1 (MQ=255)
               gCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCATgcgc  <  1:387568/61‑1 (MQ=255)
               gCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCATgcgc  <  1:929048/61‑1 (MQ=255)
               gCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCATgcgc  <  1:1734893/61‑1 (MQ=255)
               gCCACCTCTTCCTGACACTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCATgcgc  <  1:135726/61‑1 (MQ=255)
                               |                                            
TCGTAGATGGCTTTCGCCACCTCTTCCTGACGCTTCACCTGAGCTTTCATCTGGATGGTACGCGCCGCCCATGCGC  >  minE/2428921‑2428996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A