Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I2 R1 52 300.6 3297834 97.8% 3225281 57.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,772,745 T→C Y147H (TAT→CAT)  aceA → isocitrate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,772,7450TC100.0% 548.2 / NA 141Y147H (TAT→CAT) aceAisocitrate lyase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (92/49);  total (92/49)

Only 100 of 141 total aligned reads displayed.

GTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTG  >  minE/2772692‑2772793
                                                     |                                                
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:1368172/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:2404544/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:160603/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:2602693/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:2744377/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:2835524/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:1501141/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:1489340/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:2999929/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:1433105/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:1897179/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:1329607/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:3217262/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:646101/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:95757/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:180862/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:885520/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:859129/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:735113/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:72625/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:126905/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:1273718/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:1283804/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:704103/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:388799/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGGCGa                                           <  1:1442044/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTCCGCGGACATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:2971174/61‑1 (MQ=255)
gtcgtGCCGATCAGATCCAATGGTACGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:3088584/61‑1 (MQ=255)
         aTCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:2945279/52‑1 (MQ=255)
                         cgcgGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:1799204/36‑1 (MQ=255)
                         cgcgGGCATTGAGCCGGGCGATCCGCGCCATGTCGa                                           <  1:2465439/36‑1 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGaa            >  1:3066421/1‑52 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGtttt   >  1:934399/1‑61 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGtttt   >  1:967441/1‑61 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:3150126/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:423591/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:422478/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1297559/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:372230/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:3258278/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1324255/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:3204105/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:3164348/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:477559/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:3098250/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1396476/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:3075662/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1408528/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:3028910/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1462856/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1771498/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:2974260/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:491829/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:519054/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:613759/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:629618/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1291905/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1288647/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:121183/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1132775/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:779066/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1108920/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1104056/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:912164/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1049321/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1030357/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1020961/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1609477/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1733946/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1831554/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1710784/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1961455/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1996696/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:2053587/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:2058405/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:2090489/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:2097917/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:2159933/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:2181013/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:2328067/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:2385307/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:161883/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:2445167/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:2732490/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1507103/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:2944105/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:292632/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:2888128/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:2866104/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1564874/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:2786638/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1572355/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1468858/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:2688916/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:2670939/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:2654412/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:2614044/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:1587062/1‑62 (MQ=255)
                                        gggCGATCCGCGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:2581188/1‑62 (MQ=255)
                                        gggCGATCCGAGCCATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTg  >  1:664997/1‑62 (MQ=255)
                                                     |                                                
GTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTG  >  minE/2772692‑2772793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A