minE Evolution: EZR_Evolution
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Mutations |
Converged Mutations |
Fixed Mutations |
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Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A5 F28 I2 R2 | 154 | 32.4 | 3140800 | 76.4% | 2399571 | 54.5 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 547,835 | +G | pseudogene (255/261 nt) pseudogene (255/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 547,831 | 1 | . | G | 100.0% | 139.6 / NA | 40 | pseudogene (251/261 nt) pseudogene (251/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (27/13); total (27/13) | |||||||||||
TCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGC > W3110S.gb/547778‑547869 | tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg < 1:2591878/57‑1 (MQ=255)tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg < 1:2520940/57‑1 (MQ=255) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTg > 1:1217505/1‑57 (MQ=39) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTg > 1:676943/1‑57 (MQ=39) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTg > 1:2986043/1‑57 (MQ=39) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTg > 1:2605462/1‑57 (MQ=39) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTg > 1:2459402/1‑57 (MQ=39) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTg > 1:1455531/1‑57 (MQ=39) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTg > 1:1240747/1‑57 (MQ=39) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTg > 1:1364522/1‑57 (MQ=39) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTg > 1:1393189/1‑57 (MQ=39) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACtt > 1:859568/1‑62 (MQ=255) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACtt > 1:522972/1‑62 (MQ=255) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACtt > 1:2778443/1‑62 (MQ=255) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACtt > 1:224470/1‑62 (MQ=255) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACtt > 1:1438949/1‑62 (MQ=255) aGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTc < 1:930128/61‑1 (MQ=255) aGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTc < 1:690541/61‑1 (MQ=255) aGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTc < 1:471866/61‑1 (MQ=255) aGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTc < 1:3105714/61‑1 (MQ=255) aGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTc < 1:2878785/61‑1 (MQ=255) aGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTc < 1:1465927/61‑1 (MQ=255) aGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTc < 1:1871570/61‑1 (MQ=255) aGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTc < 1:1619604/61‑1 (MQ=255) ggTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTc < 1:1188473/60‑1 (MQ=255) aaaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCa < 1:2089512/62‑1 (MQ=255) aaaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCa < 1:3022216/62‑1 (MQ=255) aaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCa < 1:44085/61‑1 (MQ=255) gcgcAGGCGCTGGCAATACTGGGGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:401582/1‑62 (MQ=255) gcgcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCAcc > 1:1578991/1‑55 (MQ=255) gcgcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:1653201/1‑62 (MQ=255) gcgcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:3036203/1‑62 (MQ=255) gcgcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:1646001/1‑62 (MQ=255) gcgcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:457781/1‑62 (MQ=255) gcgcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:1681213/1‑62 (MQ=255) gcgcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:671510/1‑62 (MQ=255) gcgcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:2777877/1‑62 (MQ=255) gcgcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:1571748/1‑62 (MQ=255) gcgcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:1448469/1‑62 (MQ=255) gcgcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGa > 1:1479783/1‑61 (MQ=255) cAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGc > 1:2953412/1‑62 (MQ=255) aaTACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCa < 1:3019040/39‑1 (MQ=38) | TCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGC > W3110S.gb/547778‑547869 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |