Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,055,632 |
C→T |
A9A (GCG→GCA) |
yrhB ← |
hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,055,632 | 0 | C | T | 100.0%
| 115.5
/ NA
| 36 | A9A (GCG→GCA) | yrhB | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (10/26); total (10/26) |
CATGTAGAGTAATGACGACCGGGAGATCTGCATCATCAAGGTAATGGTTCGCTTTCGCGAATGCGTCGTGATAAGTAATCATAATTATAATAAATATTATTGTTGAGTG > W3110S.gb/4055577‑4055685
|
cATGTAGAGTAATGACGACCGGGAGATCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAAt < 1:2125788/62‑1 (MQ=255)
agTAATGACGACCGGGAGATCTGCATCATCAAGGTAATGGTTCGCTTTtgcg > 1:366878/1‑52 (MQ=255)
tAATGACGACCGGGAGATCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAAt < 1:2863703/53‑1 (MQ=255)
acgacCGGGAGATCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGt < 1:929382/62‑1 (MQ=255)
tgaTCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAAt < 1:2081641/61‑1 (MQ=255)
tgaTCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAAt < 1:2593519/61‑1 (MQ=255)
tgaTCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAAt < 1:2950604/61‑1 (MQ=255)
tgaTCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAAt < 1:2372995/61‑1 (MQ=255)
tgaTCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAAt < 1:2350409/61‑1 (MQ=255)
tgaTCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAAt < 1:3023065/61‑1 (MQ=255)
tgaTCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAAt < 1:2178625/61‑1 (MQ=255)
tgaTCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAAt < 1:2139446/61‑1 (MQ=255)
tgaTCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAAt < 1:1875028/61‑1 (MQ=255)
tgaTCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAAt < 1:186859/61‑1 (MQ=255)
tgaTCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAAt < 1:843144/61‑1 (MQ=255)
tgaTCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAAt < 1:893283/61‑1 (MQ=255)
tgaTCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAAt < 1:1752931/61‑1 (MQ=255)
tgaTCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAAt < 1:1745954/61‑1 (MQ=255)
tgaTCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAAt < 1:1742119/61‑1 (MQ=255)
tgaTCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAAt < 1:163538/61‑1 (MQ=255)
tgaTCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAAt < 1:160374/61‑1 (MQ=255)
tgaTCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAAt < 1:1484770/61‑1 (MQ=255)
tgaTCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAATGCGGCGTGATAAGTAATCATAAt < 1:2578113/61‑1 (MQ=255)
gaTCTGCATCATCAAGGTAATGGTTCTCTTTTGCGAATGCGTCGTGATAAGTAATCATAAt < 1:2808197/61‑1 (MQ=255)
gaTCTGCATCATCAAGGGAATGGATCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAAt < 1:152088/61‑1 (MQ=255)
aTCTGCATCATCAAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAAt < 1:2304430/60‑1 (MQ=255)
atcaAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAATta > 1:996404/1‑53 (MQ=255)
atcaAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAATTATaataaat > 1:2396071/1‑61 (MQ=255)
atcaAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAATTATaataaat > 1:136452/1‑61 (MQ=255)
atcaAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAATTATaataaat > 1:2245778/1‑61 (MQ=255)
atcaAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAATTATaataaat > 1:394067/1‑61 (MQ=255)
atcaAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAATTATaataaat > 1:812908/1‑61 (MQ=255)
atcaAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAATTATaataaat > 1:1823060/1‑61 (MQ=255)
atcaAGGTAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAATTATaataaa > 1:179262/1‑60 (MQ=255)
tAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAATTATAATAAATATTAttg > 1:484623/1‑61 (MQ=255)
tAATGGTTCGCTTTTGCGAATGCGTCGTGATAAGTAATCATAATTATAATAAATATTAttg > 1:963057/1‑61 (MQ=255)
cGCTTTTGCGAATGCGTCGTGATAAGTAATCATAATTATAATAAATATTATTGTTGAgtg < 1:158895/60‑1 (MQ=255)
|
CATGTAGAGTAATGACGACCGGGAGATCTGCATCATCAAGGTAATGGTTCGCTTTCGCGAATGCGTCGTGATAAGTAATCATAATTATAATAAATATTATTGTTGAGTG > W3110S.gb/4055577‑4055685
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
