Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,225,554 |
G→A |
Q176* (CAA→TAA) |
arpA ← |
regulator of acetyl CoA synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,225,554 | 0 | G | A | 100.0%
| 87.3
/ NA
| 28 | Q176* (CAA→TAA) | arpA | regulator of acetyl CoA synthetase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/12); total (16/12) |
TTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATT > W3110S.gb/4225497‑4225598
|
ttttGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTAATgg < 1:408717/62‑1 (MQ=255)
ttttGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTAATgg < 1:1596592/62‑1 (MQ=255)
cGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATCAACATTCTGAt < 1:677361/62‑1 (MQ=255)
cGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATCAACATTCTGAt < 1:3014038/62‑1 (MQ=255)
cGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATCAACATTCTGAt < 1:2693003/62‑1 (MQ=255)
cGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATCAACATTCTGAt < 1:2689373/62‑1 (MQ=255)
cGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATCAACATTCTGAt < 1:637177/62‑1 (MQ=255)
cGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATCAACATTCTGAt < 1:692110/62‑1 (MQ=255)
cGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATCAACATTCTGAt < 1:2283337/62‑1 (MQ=255)
cGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATCAACATTCTGAt < 1:1585762/62‑1 (MQ=255)
aGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATc > 1:2837461/1‑36 (MQ=255)
aGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATc > 1:615272/1‑36 (MQ=255)
aGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATc > 1:716470/1‑36 (MQ=255)
aGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATc > 1:446778/1‑36 (MQ=255)
aGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATc > 1:427202/1‑36 (MQ=255)
aGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATc > 1:920365/1‑36 (MQ=255)
aGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATc > 1:988909/1‑36 (MQ=255)
aGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATc > 1:389286/1‑36 (MQ=255)
aGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATc > 1:128906/1‑36 (MQ=255)
aGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATc > 1:2707937/1‑36 (MQ=255)
aGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATc > 1:2436936/1‑36 (MQ=255)
aGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATc > 1:239536/1‑36 (MQ=255)
aGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATc > 1:1375685/1‑36 (MQ=255)
aGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATc > 1:1342010/1‑36 (MQ=255)
aGCTGAAGAGTAATCATCTTAATGGGGTTTGTAATc > 1:1306329/1‑36 (MQ=255)
gCTGAAGAGTAATCATCTTAATGGGGTTTGTAATCAACATTCTGAt < 1:2513634/46‑1 (MQ=255)
cTGAAGAGTAATCATCTTAATGGGGTTTGTAATCAACATTCTGATAAATATCATTAatata > 1:2672994/1‑61 (MQ=255)
gAAGAGTAATCATCTTAATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATAtt < 1:913500/61‑1 (MQ=255)
|
TTTTGCATTTTTTGTTGAAGTGCGTGAGAAATTTTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATT > W3110S.gb/4225497‑4225598
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
