Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,269,081 |
2 bp→CC |
coding (1176‑1177/1416 nt) |
dnaB → |
replicative DNA helicase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,269,081 | 0 | T | C | 77.8%
| 31.5
/ 4.3
| 18 | R392R (CGT→CGC) | dnaB | replicative DNA helicase |
| Reads supporting (aligned to +/- strand): ref base T (0/4); new base C (0/14); total (0/18) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| * | W3110S.gb | 4,269,082 | 0 | G | C | 77.8%
| 33.7
/ 4.3
| 18 | A393P (GCC→CCC) | dnaB | replicative DNA helicase |
| Reads supporting (aligned to +/- strand): ref base G (0/4); new base C (0/14); total (0/18) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAAAGAACTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTAT > W3110S.gb/4269021‑4269128
||
gAAAGAACTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:2879612/61‑1 (MQ=255)
aCTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:2252961/55‑1 (MQ=255)
aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:949895/51‑1 (MQ=255)
aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:136822/51‑1 (MQ=255)
aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:137396/51‑1 (MQ=255)
aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:2842087/51‑1 (MQ=255)
aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:1666900/51‑1 (MQ=255)
aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:1794319/51‑1 (MQ=255)
aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:2754677/51‑1 (MQ=255)
aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:2503407/51‑1 (MQ=255)
aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:1891975/51‑1 (MQ=255)
aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:2471017/51‑1 (MQ=255)
aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:2400806/51‑1 (MQ=255)
aCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:2907715/50‑1 (MQ=255)
ggCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTcc < 1:1232897/61‑1 (MQ=255)
ggCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTcc < 1:218905/61‑1 (MQ=255)
ggCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTcc < 1:1798107/61‑1 (MQ=255)
ggCGCTGGCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTcc < 1:2718774/61‑1 (MQ=255)
tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:967102/59‑1 (MQ=255)
tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:1156263/59‑1 (MQ=255)
tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:940666/59‑1 (MQ=255)
tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:1339546/59‑1 (MQ=255)
tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:29036/59‑1 (MQ=255)
tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:1452226/59‑1 (MQ=255)
tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:1537191/59‑1 (MQ=255)
tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:2702613/59‑1 (MQ=255)
tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:2657334/59‑1 (MQ=255)
tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:1857772/59‑1 (MQ=255)
tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:2481426/59‑1 (MQ=255)
tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:2057201/59‑1 (MQ=255)
tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:2059971/59‑1 (MQ=255)
tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:2072536/59‑1 (MQ=255)
||
GAAAGAACTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTAT > W3110S.gb/4269021‑4269128
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
