Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R2	90	19.5	1203533	67.3%	809977	57.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,270,387	+G	coding (638/816 nt)	yedP →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,270,384	1	.	G	76.3%	323.6 / 93.2	114	coding (635/816 nt)	yedP	conserved hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/27); new base G (0/87); total (0/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

GCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGA  >  minE/1270323‑1270426

gCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑t                                             >  1:115736/1‑62 (MQ=255)

gCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑t                                             >  1:121417/1‑62 (MQ=255)

gCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑t                                             >  1:221121/1‑62 (MQ=255)

gCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑t                                             >  1:586972/1‑62 (MQ=255)

                    ctttCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:168713/59‑1 (MQ=255)

                    cTATCAACAATTGTCGGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:1123339/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATTGGCCAAACGAt                         <  1:1000987/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:213373/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:221809/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:544628/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:263134/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:275655/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:276004/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:281159/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:708829/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:315786/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:194640/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:326240/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:36094/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:366731/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:386029/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:664954/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:427744/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:437950/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:446596/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:647481/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:607142/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:566712/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:49500/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:501681/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:1021059/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:879721/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:1087068/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:109084/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:854898/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:109628/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:810419/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:1053403/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:1112745/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:112073/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:1131718/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:1135178/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:794403/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:787859/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:1192860/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:1020673/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:769925/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:905840/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:138572/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:170213/62‑1 (MQ=255)

                    cTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:764659/62‑1 (MQ=255)

                     tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:983482/61‑1 (MQ=255)

                     tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:547108/61‑1 (MQ=255)

                     tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:879791/61‑1 (MQ=255)

                     tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:684798/61‑1 (MQ=255)

                     tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:80246/61‑1 (MQ=255)

                     tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:697792/61‑1 (MQ=255)

                     tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:712559/61‑1 (MQ=255)

                     tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:715955/61‑1 (MQ=255)

                     tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:744355/61‑1 (MQ=255)

                     tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:766002/61‑1 (MQ=255)

                     tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:1008893/61‑1 (MQ=255)

                     tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:490829/61‑1 (MQ=255)

                     tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:1190574/61‑1 (MQ=255)

                     tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:1135616/61‑1 (MQ=255)

                     tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:179532/61‑1 (MQ=255)

                     tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:1104415/61‑1 (MQ=255)

                     tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:1056971/61‑1 (MQ=255)

                     tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:309582/61‑1 (MQ=255)

                     tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:1065463/61‑1 (MQ=255)

                     tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:1095788/61‑1 (MQ=255)

                     tATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:40386/61‑1 (MQ=255)

                     tATCAACAATTGTCAGACAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:837092/61‑1 (MQ=255)

                     tATCAACAATTGTCACGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:121188/61‑1 (MQ=255)

                      aTCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:273976/60‑1 (MQ=255)

                       tCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:608257/59‑1 (MQ=255)

                                gTCAGGCAAACGCCCAACCACACTTGGCC‑TGGGGCGATGGGCCAAACGAt                         <  1:951989/50‑1 (MQ=255)

                                     ccAAACGCCCAACCACACTTGGCCGG‑GGGCGATGGGCCAAACGAt                         <  1:298294/44‑1 (MQ=255)

                                        aaCGCCCAACCACACTTGGCCGG‑GGGCGATGGGCCAAACGAt                         <  1:496333/42‑1 (MQ=255)

                                          cGCCCAACCATACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa  <  1:636999/62‑1 (MQ=255)

                                          cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa  <  1:241620/62‑1 (MQ=255)

                                          cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa  <  1:819919/62‑1 (MQ=255)

                                          cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa  <  1:1107999/62‑1 (MQ=255)

                                          cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa  <  1:1012164/62‑1 (MQ=255)

                                          cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa  <  1:906473/62‑1 (MQ=255)

                                          cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa  <  1:170475/62‑1 (MQ=255)

                                          cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa  <  1:765493/62‑1 (MQ=255)

                                          cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa  <  1:268539/62‑1 (MQ=255)

                                          cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa  <  1:70652/62‑1 (MQ=255)

                                          cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa  <  1:401922/62‑1 (MQ=255)

                                          cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa  <  1:681190/62‑1 (MQ=255)

                                          cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa  <  1:661590/62‑1 (MQ=255)

                                          cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa  <  1:466292/62‑1 (MQ=255)

                                          cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa  <  1:631065/62‑1 (MQ=255)

                                          cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa  <  1:477627/62‑1 (MQ=255)

                                          cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa  <  1:580042/62‑1 (MQ=255)

                                          cGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa  <  1:556569/62‑1 (MQ=255)

                                          cGCCCAACCACACTTGGCC‑G‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa  <  1:808970/62‑1 (MQ=255)

                                           gCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGa  <  1:489765/61‑1 (MQ=255)

                                                       ttGGCC‑T‑GGGCGATGGGCCAAACGGTGCGCCCTTACTGGAGGTAATGGa  <  1:944690/49‑1 (MQ=255)

GCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAACCACACTTGGCC‑T‑GGGCGATGGGCCAAACGATGCGCCCTTACTGGAGGTAATGGA  >  minE/1270323‑1270426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A