Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I1 R2
|
90 |
19.5 |
1203533 |
67.3% |
809977 |
57.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,300,589 |
G→A |
T179I (ACC→ATC) |
yeeA ← |
conserved inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,300,589 | 0 | G | A | 100.0%
| 21.3
/ NA
| 8 | T179I (ACC→ATC) | yeeA | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (8/0); total (8/0) |
ATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATG > minE/1300570‑1300624
|
aTAGACCCGATTATACTCGATCGGACTTTTCGCCAGTTGAATGCGCCAGTGGATg > 1:128838/1‑55 (MQ=255)
aTAGACCCGATTATACTCGATCGGACTTTTCGCCAGTTGAATGCGCCAGTGGATg > 1:198168/1‑55 (MQ=255)
aTAGACCCGATTATACTCGATCGGACTTTTCGCCAGTTGAATGCGCCAGTGGATg > 1:473965/1‑55 (MQ=255)
aTAGACCCGATTATACTCGATCGGACTTTTCGCCAGTTGAATGCGCCAGTGGATg > 1:634322/1‑55 (MQ=255)
aTAGACCCGATTATACTCGATCGGACTTTTCGCCAGTTGAATGCGCCAGTGGATg > 1:668099/1‑55 (MQ=255)
aTAGACCCGATTATACTCGATCGGACTTTTCGCCAGTTGAATGCGCCAGTGGATg > 1:703840/1‑55 (MQ=255)
aTAGACCCGATTATACTCGATCGGACTTTTCGCCAGTTGAATGCGCCAGTGGATg > 1:733957/1‑55 (MQ=255)
aTAGACCCGATTATACTCGATCGGACTTTTCGCCAGTTGAATGCGCCAGTGGATg > 1:890399/1‑55 (MQ=255)
|
ATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATG > minE/1300570‑1300624
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
