Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I1 R2
|
90 |
19.5 |
1203533 |
67.3% |
809977 |
57.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,445,058 |
T→G |
G26G (GGA→GGC) |
ilvD ← |
dihydroxyacid dehydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,445,058 | 0 | T | G | 100.0%
| 15.9
/ NA
| 6 | G26G (GGA→GGC) | ilvD | dihydroxyacid dehydratase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (0/6); total (0/6) |
GATAATCGGCTTACCGAAATCGGCGTCGGTCATTCCGGTGGCGCGCCACAGCGCACGAGCAC > minE/2445025‑2445086
|
gatgatCGGTTTCTCGAAGTCGCCGTCCTTCATGCCGGTGGCGCGCCACAGGGCGCGGGctc < 1:236511/62‑3 (MQ=255)
gatgatCGGTTTCTCGAAGTCGCCGTCCTTCATGCCGGTGGCGCGCCACAGGGCGCGGGctc < 1:447497/62‑3 (MQ=255)
gatgatCGGTTTCTCGAAGTCGCCGTCCTTCATGCCGGTGGCGCGCCACAGGGCGCGGGctc < 1:459721/62‑3 (MQ=255)
gatgatCGGTTTCTCGAAGTCGCCGTCCTTCATGCCGGTGGCGCGCCACAGGGCGCGGGctc < 1:466005/62‑3 (MQ=255)
gatgatCGGTTTCTCGAAGTCGCCGTCCTTCATGCCGGTGGCGCGCCACAGGGCGCGGGctc < 1:630416/62‑3 (MQ=255)
gatgatCGGTTTCTCGAAGTCGCCGTCCTTCATGCCGGTGGCGCGCCACAGGGCGCGGGctc < 1:925371/62‑3 (MQ=255)
|
GATAATCGGCTTACCGAAATCGGCGTCGGTCATTCCGGTGGCGCGCCACAGCGCACGAGCAC > minE/2445025‑2445086
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
