Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I3 R1 178 99.0 1887089 95.3% 1798395 63.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,863,108 T→C L73L (TTA→CTA)  ygbM → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,863,1080TC100.0% 437.0 / NA 114L73L (TTA→CTA) ygbMconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/67);  total (47/67)

Only 100 of 114 total aligned reads displayed.

ACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAA  >  W3110S.gb/2863072‑2863167
                                    |                                                           
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGc                                                      >  1:343801/1‑44 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACg                                  >  1:1827862/1‑64 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACg                                  >  1:1544081/1‑64 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACg                                  >  1:199150/1‑64 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACg                                  >  1:1455739/1‑64 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:1862018/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:746069/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:688675/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:458379/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:1666284/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:1677354/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:1012108/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:548979/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:211825/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:544270/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:1707061/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:1712511/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:542873/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:1737615/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:1743697/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:1746259/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:536440/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:51309/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:1830210/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:211647/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:1200099/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:1211397/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:1227044/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:1296804/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:1310404/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:1317700/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:1323363/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:854202/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:777812/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:375978/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:941159/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:24266/1‑65 (MQ=255)
aCCGCTCCCGGAGATATTAATGCGGGGGAACGGGGACTATCCGCCCTTCCCGGACGTGAGCACGa                                 >  1:731822/1‑65 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCTCGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1849511/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGTCACTGGaa  <  1:855732/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:393795/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:402351/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:435640/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:437682/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:439764/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:450791/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:646302/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:766436/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:946940/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:89690/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:867613/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:862825/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:806230/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:780951/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:775022/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:772598/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:471128/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:765329/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:759679/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:682886/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:668800/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:192133/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:548860/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:532955/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1466431/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:377806/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1606564/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1596684/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1564481/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:156274/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1528529/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1527903/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1505618/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1611633/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1451191/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1451089/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1349059/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1197181/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1151317/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:115036/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1052932/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1024602/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1819982/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:355257/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:349543/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:320626/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:26896/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:263046/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:197984/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1829196/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1628300/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1791589/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1748549/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1718858/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1685741/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1679605/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1678163/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1636908/64‑1 (MQ=255)
                                gggACTATCCGCCCTTCCCGGACGTGAGAACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1699486/64‑1 (MQ=255)
                                gggACTATCCGCCATTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa  <  1:1221282/64‑1 (MQ=255)
                                    |                                                           
ACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAA  >  W3110S.gb/2863072‑2863167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A