Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I3 R1
|
178 |
99.0 |
1887089 |
95.3% |
1798395 |
63.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,030,382 |
+G |
coding (360/1449 nt) |
ygfU → |
predicted transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,030,378 | 1 | . | G | 100.0%
| 19.4
/ NA
| 7 | L119R (CTG→CGG) | ygfU | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (7/0); total (7/0) |
GGGATGAACCCGGATATCGGCCTGCT‑GGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATC > W3110S.gb/3030353‑3030415
|
gggATGAACCCGGATATCGGCCTGCTGGGGGATATTTGGt > 1:337210/1‑40 (MQ=37)
gggATGAACCCGGATATCGGCCTGCTGGGGGATATTTGGTGCCACTATCGCCGCGGGtttttta > 1:945213/1‑61 (MQ=255)
gggATGAACCCGGATATCGGCCTGCTGGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATc > 1:1635482/1‑64 (MQ=255)
gggATGAACCCGGATATCGGCCTGCTGGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATc > 1:1713829/1‑64 (MQ=255)
gggATGAACCCGGATATCGGCCTGCTGGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATc > 1:228543/1‑64 (MQ=255)
gggATGAACCCGGATATCGGCCTGCTGGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATc > 1:249331/1‑64 (MQ=255)
gggATGAACCCGGATATCGGCCTGCTGGGGGATATTTGGTGCCACTATCGCCGCGGGGTTTAta > 1:1720849/1‑63 (MQ=255)
|
GGGATGAACCCGGATATCGGCCTGCT‑GGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATC > W3110S.gb/3030353‑3030415
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
