Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I1 R1 354 41.7 3110465 93.1% 2895842 61.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,538,270 A→G F21S (TTT→TCT)  narV ← nitrate reductase 2 (NRZ), gamma subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,538,2700AG100.0% 232.4 / NA 61F21S (TTT→TCT) narVnitrate reductase 2 (NRZ), gamma subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/61);  total (0/61)

GGTGTACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTC  >  W3110S.gb/1538227‑1538317
                                           |                                               
ggggtACTGCCCTTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:669512/62‑1 (MQ=255)
ggTGTACTGCCCGTAGTCGTAGCGCAGCTAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:973916/65‑1 (MQ=38)
ggTGTACTGCCCGTAGTCGTAGCGCAGCTAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:970328/65‑1 (MQ=38)
ggTGTACTGCCCGTAGTCGTAGCGCAGCCTGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:81388/65‑1 (MQ=38)
ggTGTACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:1178292/65‑1 (MQ=255)
ggTGTACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:2780142/65‑1 (MQ=255)
ggTGTACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:543530/65‑1 (MQ=255)
 gtgtACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:1861043/64‑1 (MQ=255)
  tgtACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:919267/63‑1 (MQ=255)
    tACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:719910/61‑1 (MQ=255)
    tACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:2698539/61‑1 (MQ=255)
    tACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:275731/61‑1 (MQ=255)
    tACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:2101489/61‑1 (MQ=255)
    tACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:2856830/61‑1 (MQ=255)
    tACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:189751/61‑1 (MQ=255)
    tACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:1829442/61‑1 (MQ=255)
    tACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:815526/61‑1 (MQ=255)
    tACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:826803/61‑1 (MQ=255)
    tACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:827162/61‑1 (MQ=255)
    tACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:852289/61‑1 (MQ=255)
    tACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:920165/61‑1 (MQ=255)
    tACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:1197984/61‑1 (MQ=255)
             tAGTCGTAGGGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:1212505/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGtc               <  1:282897/65‑2 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:2592331/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:2275542/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:943081/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:891980/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:3005816/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:880397/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:635767/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:639670/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:655884/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:1927482/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:1393005/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:1413128/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:1434937/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:1255339/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:1245892/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:1794997/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:116089/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:2041890/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:2185548/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:1010997/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:2386486/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:1062361/65‑1 (MQ=255)
             tAGTCGTAGCGCAGCCACCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTa               <  1:1446334/65‑1 (MQ=255)
                       gcAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACg     <  1:1961069/65‑1 (MQ=255)
                       gcAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACg     <  1:1434375/65‑1 (MQ=255)
                         agccagcTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGtt   <  1:1079138/65‑1 (MQ=255)
                         agccagcTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGtt   <  1:2072660/65‑1 (MQ=255)
                         agccagcTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGtt   <  1:1482368/65‑1 (MQ=255)
                          gccagcTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTc  <  1:685468/65‑1 (MQ=255)
                          gccagcTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTc  <  1:1070567/65‑1 (MQ=255)
                          gccagcTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTc  <  1:1375894/65‑1 (MQ=255)
                          gccagcTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTc  <  1:1654682/65‑1 (MQ=255)
                          gccagcTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTc  <  1:1878266/65‑1 (MQ=255)
                          gccagcTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTc  <  1:351232/65‑1 (MQ=255)
                          gccagcTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTc  <  1:221268/65‑1 (MQ=255)
                          gccagcTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTc  <  1:2435399/65‑1 (MQ=255)
                           ccagcTGCCGAGGAAAGACACCGTCGCACAAATGTACg                            <  1:2518214/38‑1 (MQ=255)
                                           |                                               
GGTGTACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTC  >  W3110S.gb/1538227‑1538317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A