Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,295,693 |
C→T |
A18T (GCG→ACG) |
ccmH ← |
heme lyase, CcmH subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,295,693 | 0 | C | T | 100.0%
| 166.4
/ NA
| 51 | A18T (GCG→ACG) | ccmH | heme lyase, CcmH subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (23/28); total (23/28) |
AGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGCCAGCGCTGAGCCGGAGATCAT > W3110S.gb/2295631‑2295714
|
aGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCa > 1:435937/1‑65 (MQ=255)
aGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCa > 1:2583628/1‑65 (MQ=255)
aGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCa > 1:2474318/1‑65 (MQ=255)
aGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCa > 1:264736/1‑65 (MQ=255)
aGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCa > 1:2803931/1‑65 (MQ=255)
aGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCa > 1:2147894/1‑65 (MQ=255)
aGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCa > 1:2879373/1‑65 (MQ=255)
aGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCa > 1:1973227/1‑65 (MQ=255)
aGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCa > 1:1562358/1‑65 (MQ=255)
aGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCa > 1:1191175/1‑65 (MQ=255)
aGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCa > 1:942613/1‑65 (MQ=255)
aGTGAGCAGACGGAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCa > 1:82534/1‑65 (MQ=255)
ggAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGct < 1:318058/64‑2 (MQ=255)
ggAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGcc < 1:1523072/64‑1 (MQ=255)
ggAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGcc < 1:1092138/64‑1 (MQ=255)
ggAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGcc < 1:999162/64‑1 (MQ=255)
ggAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGcc < 1:943148/64‑1 (MQ=255)
ggAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGcc < 1:2807229/64‑1 (MQ=255)
ggAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGcc < 1:1431323/64‑1 (MQ=255)
ggAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGcc < 1:3088558/64‑1 (MQ=255)
ggAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGcc < 1:888897/64‑1 (MQ=255)
ggAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGcc < 1:376827/64‑1 (MQ=255)
ggAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGcc < 1:1748962/64‑1 (MQ=255)
ggAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGcc < 1:1575861/64‑1 (MQ=255)
aCTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgag < 1:1190087/65‑1 (MQ=255)
aCTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgag < 1:2659859/65‑1 (MQ=255)
aCTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgag < 1:438987/65‑1 (MQ=255)
aCTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgag < 1:503215/65‑1 (MQ=255)
aCTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgag < 1:2375335/65‑1 (MQ=255)
aCTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgag < 1:1261734/65‑1 (MQ=255)
aCTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgag < 1:1261914/65‑1 (MQ=255)
aCTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgag < 1:1612216/65‑1 (MQ=255)
aCTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgag < 1:175371/65‑1 (MQ=255)
aCTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgag < 1:1899684/65‑1 (MQ=255)
aCTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgag < 1:2250862/65‑1 (MQ=255)
aCTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgag < 1:2344457/65‑1 (MQ=255)
aCTGTTGGTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgag < 1:636593/65‑1 (MQ=255)
cTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgag < 1:2129972/64‑1 (MQ=255)
ttgttCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGatc > 1:532714/1‑64 (MQ=255)
ttgttCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGatc > 1:2466633/1‑64 (MQ=255)
ttgttCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGatc > 1:962291/1‑64 (MQ=255)
ttgttCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGatc > 1:2726995/1‑64 (MQ=255)
ttgttCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGatc > 1:1329976/1‑64 (MQ=255)
ttgttCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGatc > 1:1526617/1‑64 (MQ=255)
ttgttCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGatc > 1:2730144/1‑64 (MQ=255)
ttgttCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGatc > 1:1640147/1‑64 (MQ=255)
ttgttCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGatc > 1:1711742/1‑64 (MQ=255)
ttgttCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGatc > 1:1717042/1‑64 (MQ=255)
ttgttCCTGTGCTTCATCTTTAAACTGCAACACATCGATGCTCGTCAGCGCTGAGCCGGAGata > 1:2001367/1‑63 (MQ=255)
gttCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGGAGatcat < 1:500847/64‑1 (MQ=255)
ccTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGTCAGCGCTGAGCCGgag < 1:659094/56‑1 (MQ=255)
|
AGTGAGCTGACGGAACTGTTGTTCCTGTGCTTCATCTTTAAACTGCAACACATCGATGGTCGCCAGCGCTGAGCCGGAGATCAT > W3110S.gb/2295631‑2295714
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
