Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I3 R1 176 0.0 1562086 58.2% 909134 66.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 289,267 C→T S637S (TCC→TCT)  lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE289,2670CT100.0% 276.1 / NA 76S637S (TCC→TCT) lonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/76);  total (0/76)

GCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTA  >  minE/289221‑289282
                                              |               
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:625810/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:165123/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:302261/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:38261/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:475293/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:49013/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:523607/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:533811/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:534392/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:539417/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:558046/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:561472/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:591961/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:592026/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:593245/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:605475/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1022059/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:637358/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:645451/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:653729/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:660110/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:707099/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:714001/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:775986/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:813181/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:834947/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:86858/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:90490/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:917988/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:937879/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:985574/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:996290/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1156793/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:132446/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1312765/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1299378/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:162115/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1265238/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1256191/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1247102/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1207546/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1294203/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1147399/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1136987/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1110564/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1100171/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1061078/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1027787/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1022593/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1011969/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1477456/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:158467/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1548917/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1514047/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1328535/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1450946/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1442664/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1409993/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1433612/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1406000/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1400732/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:136733/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGCTa  <  1:665192/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGCTa  <  1:666868/60‑1 (MQ=255)
gtaaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCCGCGTTa  <  1:199362/60‑1 (MQ=255)
 taaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGTCGTTa  <  1:196549/60‑1 (MQ=255)
 taaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1342334/60‑1 (MQ=255)
 taaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:246557/60‑1 (MQ=255)
 taaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:874724/60‑1 (MQ=255)
 taaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:337614/60‑1 (MQ=255)
 taaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:845088/60‑1 (MQ=255)
 taaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:837278/60‑1 (MQ=255)
 taaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:832870/60‑1 (MQ=255)
 taaaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:536175/60‑1 (MQ=255)
  aaaCTGAGCTATACCGGTTCGCTCGGCGAAGTGATGGAGGAGTCTATTCAGGCGGCGTTa  <  1:882236/60‑1 (MQ=255)
                        tCGGCGAAGTGATGCAGGAGTCTATTCAGGCGGCGTTa  <  1:1157947/38‑1 (MQ=255)
                                              |               
GCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTA  >  minE/289221‑289282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A