Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I3 R1 176 0.0 1562086 58.2% 909134 66.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,662,064 G→C A255A (GCC→GCG)  rtcR ← sigma 54‑dependent transcriptional regulator of rtcBA expression

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,662,0640GC100.0% 161.0 / NA 46A255A (GCC→GCG) rtcRsigma 54‑dependent transcriptional regulator of rtcBA expression
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (46/0);  total (46/0)

AACGCGCCTTTTACATGACCAAACAGCGTCGACATGGCGGTATCGCCGCGCAGGGTGGCGCAGTTCACTTC  >  minE/2662029‑2662099
                                   |                                   
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:771791/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:339710/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:35175/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:404357/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:404535/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:453722/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:455975/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:555926/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:650201/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:694692/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:730647/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:757236/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:283715/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:809739/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:824738/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:863554/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:864989/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:888574/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:898025/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:913595/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:924173/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:974876/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:997048/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1431963/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1086725/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1110253/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1131188/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1285323/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1288228/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1324965/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1342221/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1342859/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1361041/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1366329/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1417682/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1006492/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1446799/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1487482/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1542430/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:1547943/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:188011/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:195811/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:213257/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:227824/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:240710/4‑71 (MQ=255)
aaggcgcCCTTGACGTGACCGAACAGTGCCGACATCGCGCCGTCGCCGCGCAAAGTGGCGCAGTTGACCTc  >  1:274650/4‑71 (MQ=255)
                                   |                                   
AACGCGCCTTTTACATGACCAAACAGCGTCGACATGGCGGTATCGCCGCGCAGGGTGGCGCAGTTCACTTC  >  minE/2662029‑2662099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A