Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R2 146 74.7 3299319 84.8% 2797822 66.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 995,494 C→T G254G (GGG→GGA)  dgsA ← DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE995,4940CT100.0% 513.6 / NA 148G254G (GGG→GGA) dgsADNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (79/69);  total (79/69)

Only 100 of 148 total aligned reads displayed.

CTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGC  >  minE/995438‑995554
                                                        |                                                            
cTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGAc                                                <  1:2117661/71‑1 (MQ=255)
cTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGAc                                                <  1:2777555/71‑1 (MQ=255)
cTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGAc                                                <  1:2730084/71‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:1745338/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:1009105/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:568033/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:2987572/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:461412/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:3083685/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:2370836/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:2359818/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:2358715/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:3087249/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:3112687/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:2356079/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:2186851/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:2217867/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:1032738/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:1611041/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:2935816/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:2691573/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:15319/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:1487591/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:1162401/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:1163845/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:2693891/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:673503/70‑1 (MQ=255)
   tCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:1177826/70‑1 (MQ=255)
    ccACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCtgt                                            >  1:1686263/1‑71 (MQ=255)
                   tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATGCGGGTCGACCTGTGTGTGGCCTATTTc                              >  1:323878/1‑70 (MQ=255)
                   tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACgg                                                      >  1:2944274/1‑46 (MQ=255)
                   tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGCCCTGTGTGTGGCCTATTTc                              >  1:2641684/1‑70 (MQ=255)
                   tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTc                              >  1:2705787/1‑70 (MQ=255)
                   tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTc                              >  1:3039813/1‑70 (MQ=255)
                   tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTc                              >  1:3035558/1‑70 (MQ=255)
                   tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTc                              >  1:26487/1‑70 (MQ=255)
                   tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTc                              >  1:2838056/1‑70 (MQ=255)
                   tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTc                              >  1:187796/1‑70 (MQ=255)
                   tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTc                              >  1:118834/1‑70 (MQ=255)
                   tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTc                              >  1:1243475/1‑70 (MQ=255)
                   tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTc                              >  1:721634/1‑70 (MQ=255)
                   tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTc                              >  1:2258844/1‑70 (MQ=255)
                   tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTc                              >  1:833069/1‑70 (MQ=255)
                   tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTc                              >  1:430908/1‑70 (MQ=255)
                   tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTc                              >  1:378478/1‑70 (MQ=255)
                   tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTc                              >  1:1120453/1‑70 (MQ=255)
                   tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTc                              >  1:1955125/1‑70 (MQ=255)
                   tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTc                              >  1:2071208/1‑70 (MQ=255)
                   tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTc                              >  1:3159026/1‑70 (MQ=255)
                   tttCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTc                              >  1:309387/1‑70 (MQ=255)
                   tttCGAGGCAGCCGGGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTc                              >  1:3072812/1‑70 (MQ=255)
                             gCCGTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:59496/44‑1 (MQ=255)
                                gTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTActgc                >  1:2479795/1‑71 (MQ=255)
                                gTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTActgc                >  1:1745997/1‑71 (MQ=255)
                                gTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTActgc                >  1:2950416/1‑71 (MQ=255)
                                gTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTActgc                >  1:624956/1‑71 (MQ=255)
                                gTGATTCCCGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTActg                 >  1:1675740/1‑70 (MQ=255)
                                gTGATTCACGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTActgc                >  1:1823890/1‑71 (MQ=255)
                                    ttCCCGCAATAACAGCGTTTTCCATACGGGTCGACCt                                              <  1:3003780/37‑1 (MQ=255)
                                       ccGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGctgcctg          <  1:3269620/70‑1 (MQ=255)
                                       ccGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGctgcctg          <  1:1029865/70‑1 (MQ=255)
                                       ccGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGctgcctg          <  1:2880208/70‑1 (MQ=255)
                                       ccGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGctgcctg          <  1:1130387/70‑1 (MQ=255)
                                       ccGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGctgcctg          <  1:1955905/70‑1 (MQ=255)
                                       ccGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGctgcctg          <  1:2078489/70‑1 (MQ=255)
                                       ccGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGctgcctg          <  1:2671302/70‑1 (MQ=255)
                                       ccGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGctgcctg          <  1:2414783/70‑1 (MQ=255)
                                       ccGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGctgcctg          <  1:2519210/70‑1 (MQ=255)
                                       ccGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGctgcctg          <  1:1294053/70‑1 (MQ=255)
                                       ccGCAATAACAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCAAGAGACTACTGctgcctg          <  1:1881619/70‑1 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGactactta                >  1:2091595/1‑54 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGacta                    >  1:746841/1‑52 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:693416/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:650193/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:707930/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:1299650/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:1568965/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:1808600/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:1256500/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:546180/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:885006/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:477486/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:1855078/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:1899493/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:429859/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:3245549/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:3108802/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:2186056/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:2482980/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:2522992/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:2593205/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:2607726/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:2832210/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:2889057/1‑69 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGc  <  1:1892374/70‑1 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGc  <  1:559443/70‑1 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGc  <  1:2899984/70‑1 (MQ=255)
                                               aCAGCGTTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACCACTGCTGCCTGCGTGTAg   >  1:2804484/1‑69 (MQ=255)
                                               aCAGCGTTTTCAATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:235716/1‑69 (MQ=255)
                                               aCAGCGGTTTCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAg   >  1:5405/1‑69 (MQ=255)
                                                        |                                                            
CTGTCCACGCTGGCGATGGTTTCGAGGCAGCCGTGATTCCCGCAATAACAGCGTTTCCCATACGGGTCGACCTGTGTGTGGCCTATTTCCACGAGACTACTGCTGCCTGCGTGTAGC  >  minE/995438‑995554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A