Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R1
|
328 |
90.1 |
2317887 |
85.2% |
1974839 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
525,695 |
C→T |
100% |
P65S (CCG→TCG) |
moaD → |
molybdopterin synthase, small subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 525,695 | 0 | C | T | 91.6%
| 169.9
/ 4.9
| 59 | P65S (CCG→TCG) | moaD | molybdopterin synthase, small subunit |
| Reads supporting (aligned to +/- strand): ref base C (5/0); new base T (54/0); total (59/0) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GCAAATTACTGGCTGCCGTCAACCAGACGCTGGTGAGTTTTGACCATCCGCTGACTGACGGCGACGAAGTAGCTTTCTTCCCGCCGGTAACCGGA > minE/525648‑525742
|
gCAAATTACTGGCTGCCGTCACCCAGACGCTGGTGAGTTTTGACCATCCGCTGACTGACGGcgacga > 1:1551448/1‑67 (MQ=255)
gCAAATTACTGGCTGCCGTCAACCAGACGCTGGTGAGTTTTGACCATCCGCTGACTGACGGcgacga > 1:581767/1‑67 (MQ=255)
gCAAATTACTGGCTGCCGTCAACCAGACGCTGGTGAGTTTTGACCATCCGCTGACTGACGGcgacga > 1:313097/1‑67 (MQ=255)
gCAAATTACTGGCTGCCGTCAACCAGACGCTGGTGAGTTTTGACCATCCGCTGACTGACGGcgacga > 1:1472449/1‑67 (MQ=255)
gCAAATTACTGGCTGCCGTCAACCAGACGCTGGTGAGTTTTGACCATCCGCTGACTGACGGcgacga > 1:1942182/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGcgac > 1:1652737/1‑37 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTttct > 1:1952049/1‑50 (MQ=39)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTa > 1:2197006/1‑61 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCgg > 1:1349821/1‑66 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCgg > 1:438428/1‑66 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:382424/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:514468/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:2278748/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:363693/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:35120/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:334923/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:247595/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:238012/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:2171824/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:532078/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:627440/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:681850/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:706513/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:743981/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:778838/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:789795/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:796838/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:799899/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:834307/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:841748/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:878334/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:901554/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:93079/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:1456310/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:103100/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:1093394/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:1094911/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:1098840/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:1120447/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:1126192/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:1158416/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:1165349/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:1214960/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:1218728/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:122684/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:1375731/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:227453/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:1597403/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:1716885/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:1846929/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:1961647/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:2000107/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:2079440/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:2091982/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:2106580/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:2164514/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:1008981/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:222/1‑67 (MQ=255)
gCTGGTGAGTTTTGACCATTCGCTGACTGACGGCGACGAAGTGGCTTTCTTCCCGCCGGTAACCGGa > 1:226759/1‑67 (MQ=255)
|
GCAAATTACTGGCTGCCGTCAACCAGACGCTGGTGAGTTTTGACCATCCGCTGACTGACGGCGACGAAGTAGCTTTCTTCCCGCCGGTAACCGGA > minE/525648‑525742
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
