Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R1
|
328 |
90.1 |
2317887 |
85.2% |
1974839 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,719,972 |
G→A |
100% |
G41G (GGC→GGT) |
glnB ← |
regulatory protein P‑II for glutamine synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,719,972 | 0 | G | A | 100.0%
| 25.0
/ NA
| 9 | G41G (GGC→GGT) | glnB | regulatory protein P‑II for glutamine synthetase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/9); total (0/9) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
AGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCAT > minE/1719961‑1720013
|
aGCTCGGTGTGACCCTTCTGGCGACCGAAACCCTTCACTTCGGTCACCGTCAt < 1:1013692/53‑1 (MQ=255)
aGCTCGGTGTGACCCTTCTGGCGACCGAAACCCTTCACTTCGGTCACCGTCAt < 1:10463/53‑1 (MQ=255)
aGCTCGGTGTGACCCTTCTGGCGACCGAAACCCTTCACTTCGGTCACCGTCAt < 1:1277487/53‑1 (MQ=255)
aGCTCGGTGTGACCCTTCTGGCGACCGAAACCCTTCACTTCGGTCACCGTCAt < 1:1478699/53‑1 (MQ=255)
aGCTCGGTGTGACCCTTCTGGCGACCGAAACCCTTCACTTCGGTCACCGTCAt < 1:308007/53‑1 (MQ=255)
aGCTCGGTGTGACCCTTCTGGCGACCGAAACCCTTCACTTCGGTCACCGTCAt < 1:392700/53‑1 (MQ=255)
aGCTCGGTGTGACCCTTCTGGCGACCGAAACCCTTCACTTCGGTCACCGTCAt < 1:437192/53‑1 (MQ=255)
aGCTCGGTGTGACCCTTCTGGCGACCGAAACCCTTCACTTCGGTCACCGTCAt < 1:795474/53‑1 (MQ=255)
aGCTCGGTGCGACCCTTCTGGCGACCGAAACCCTTCACTTCGGTCACCGTCAt < 1:224051/53‑1 (MQ=255)
|
AGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCAT > minE/1719961‑1720013
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
