Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,234,615 |
+T |
intergenic (‑8/+138) |
dsbB ← / ← nhaB |
oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I/sodium:proton antiporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,234,612 | 1 | . | T | 83.8%
| 106.4
/ 16.2
| 37 | intergenic (‑5/+141) | dsbB/nhaB | oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I/sodium:proton antiporter |
| Reads supporting (aligned to +/- strand): ref base . (0/6); new base T (31/0); total (31/6) |
| Fisher's exact test for biased strand distribution p-value = 4.30e-07 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 |
AGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCA‑TTTCCCTGCAATA > W3110S.gb/1234561‑1234625
|
aGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCA‑TTTCCCTGCAata < 1:2086436/65‑1 (MQ=255)
aGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCA‑TTTCCCTGCAata < 1:1117857/65‑1 (MQ=255)
aGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCA‑TTTCCCTGCAata < 1:831697/65‑1 (MQ=255)
aGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCA‑TTTCCCTGCAata < 1:2200865/65‑1 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:331710/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:2086588/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:2121439/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:2121742/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:2157714/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:2205909/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:251061/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:2068511/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:422776/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:585021/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:699239/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:712532/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:828868/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:901732/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:1475727/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:1116033/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:1118375/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:1233914/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:1307736/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:1315542/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:1347865/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:138147/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:1442647/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:1646213/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:1739257/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:1797594/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:1845959/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:1988918/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAata > 1:20614/1‑65 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAat > 1:1567452/1‑64 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTTCCCTGCAat > 1:591531/1‑64 (MQ=255)
gCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCA‑TTTCCCTGCAata < 1:1100413/64‑1 (MQ=255)
aCGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCA‑TTTCCCTGCAata < 1:2261393/61‑1 (MQ=255)
|
AGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCA‑TTTCCCTGCAATA > W3110S.gb/1234561‑1234625
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
