Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,493,301 |
+A |
coding (851/1080 nt) |
frwC ← |
predicted enzyme IIC component of PTS |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,493,298 | 1 | . | A | 100.0%
| 126.4
/ NA
| 36 | C285F (TGT→TTT) | frwC | predicted enzyme IIC component of PTS |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (0/36); total (0/36) |
CCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑AAACTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGG > W3110S.gb/3493247‑3493356
|
cgccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:408222/63‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:1857007/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:343552/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:34045/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:308111/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:276201/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:268998/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:2393255/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:488411/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:2360807/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:588275/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:2180513/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:2118387/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:2015619/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:1900167/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:67335/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:833372/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:174814/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:952991/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:1616369/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:1588017/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:1430771/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:1427741/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:1415748/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:1388980/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:999808/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:1213623/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:1191324/65‑1 (MQ=255)
ccccAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:1090506/65‑1 (MQ=255)
ccTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:2396660/59‑1 (MQ=39)
cTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:1098391/58‑1 (MQ=39)
tGCATAGCTCTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGa < 1:133208/57‑1 (MQ=38)
gCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGACCATGATgg < 1:2370549/65‑1 (MQ=255)
gCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGACCATGATgg < 1:2209895/65‑1 (MQ=255)
gCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTGAACCGACCATGATgg < 1:1844757/65‑1 (MQ=255)
cATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCACAAAACTTAACCGa < 1:367541/55‑1 (MQ=38)
caccacaAAACTGAACCGACCTTGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAgg > 1:669774/1‑65 (MQ=255)
caccacaAAACTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAgg > 1:83198/1‑65 (MQ=255)
caccacaAAACTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAgg > 1:1684004/1‑65 (MQ=255)
caccacaAAACTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAg > 1:184403/1‑64 (MQ=255)
|
CCCCAGCCTGCATAGCACTGCGCACCGACCAGCGCCGCAGTTACTGCACCAC‑AAACTGAACCGACCATGATGGAAGGAATGACACGCAGCGGATCGGCAGCGGCGAAAGG > W3110S.gb/3493247‑3493356
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
