Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I3 R1
|
178 |
99.0 |
1887089 |
95.3% |
1798395 |
63.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,466,305 |
2 bp→GA |
coding (75‑76/285 nt) |
yfcZ ← |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,466,305 | 0 | A | G | 100.0%
| 190.5
/ NA
| 51 | C26R (TGC→CGC) | yfcZ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (51/0); total (51/0) |
| * | W3110S.gb | 2,466,306 | 0 | G | A | 100.0%
| 186.3
/ NA
| 51 | D25D (GAC→GAT) | yfcZ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (51/0); total (51/0) |
CTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATG > W3110S.gb/2466260‑2466324
||
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAg > 1:735015/1‑53 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:59147/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:258662/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:299197/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:303556/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:34048/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:365370/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:407956/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:419883/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:435930/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:466856/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:525255/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:553788/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:565498/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:247088/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:601244/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:630879/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:632852/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:637120/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:710683/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:783894/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:796854/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:833115/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:860556/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:968173/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:979426/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:1470359/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:1107609/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:1185680/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:121099/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:1220703/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:1232288/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:1259852/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:1272032/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:1280010/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:1284954/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:132942/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:1377400/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:1427246/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:1046829/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:1492586/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:1517673/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:1549794/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:155291/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:1759815/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:1764858/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:1786365/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:1796155/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:188192/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:192247/1‑65 (MQ=255)
ctGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCGATCGGAGTTGTCCATAATg > 1:209388/1‑65 (MQ=255)
||
CTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATG > W3110S.gb/2466260‑2466324
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
