Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
712,019 |
G→A |
intergenic (‑132/+8) |
fldA ← / ← ybfE |
flavodoxin 1/LexA regulated protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 712,019 | 0 | G | A | 100.0%
| 167.4
/ NA
| 53 | intergenic (‑132/+8) | fldA/ybfE | flavodoxin 1/LexA regulated protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (46/7); total (46/7) |
CAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTC > W3110S.gb/711967‑712077
|
cAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTaa > 1:1789382/1‑64 (MQ=255)
cAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTaa > 1:2768035/1‑64 (MQ=255)
cAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACg < 1:793318/64‑1 (MQ=255)
cAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACg < 1:1839217/64‑1 (MQ=255)
cAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACg < 1:688645/64‑1 (MQ=255)
cAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACg < 1:1610988/64‑1 (MQ=255)
cAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACg < 1:3104305/64‑1 (MQ=255)
cAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACg < 1:2210597/64‑1 (MQ=255)
cAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACg < 1:2404923/64‑1 (MQ=255)
cTGTGCTACATGAAAATGGAAGTTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa > 1:430940/1‑64 (MQ=255)
cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCt > 1:1440538/1‑37 (MQ=255)
cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa > 1:1154555/1‑64 (MQ=255)
cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa > 1:2821125/1‑64 (MQ=255)
cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa > 1:2878938/1‑64 (MQ=255)
cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa > 1:2067499/1‑64 (MQ=255)
cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa > 1:37796/1‑64 (MQ=255)
cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa > 1:2002442/1‑64 (MQ=255)
cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa > 1:610492/1‑64 (MQ=255)
cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa > 1:713922/1‑64 (MQ=255)
cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa > 1:1834767/1‑64 (MQ=255)
cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa > 1:1820513/1‑64 (MQ=255)
cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa > 1:1079417/1‑64 (MQ=255)
cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa > 1:1226118/1‑64 (MQ=255)
cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa > 1:1421601/1‑64 (MQ=255)
cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa > 1:1472579/1‑64 (MQ=255)
cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCAAGTTGCTGCa > 1:2565536/1‑64 (MQ=255)
cTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCAGCCAGTtgctgc > 1:521159/1‑63 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGcgccgc > 1:1799103/1‑44 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:1412181/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:970051/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:1869645/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:1910892/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:1245108/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:1248670/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:192836/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:453144/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:1288257/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:415944/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:1311473/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:1408328/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:3103101/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:2098368/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:1628466/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:2802929/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:278447/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:1582860/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:2699556/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:2670563/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:2094178/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:1520612/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:2396189/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:1540271/1‑65 (MQ=255)
aTGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtctc > 1:2102359/1‑65 (MQ=255)
|
CAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTC > W3110S.gb/711967‑712077
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
