Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R2
|
278 |
43.2 |
2215644 |
94.6% |
2095999 |
61.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,215,064 |
G→A |
20.1% |
L291L (CTG→CTA) |
yebQ → |
predicted transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,215,064 | 0 | G | A | 20.1%
| 97.5
/ 19.3
| 45 | L291L (CTG→CTA) | yebQ | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base G (22/13); new base A (3/6); total (25/20) |
| Fisher's exact test for biased strand distribution p-value = 1.44e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTCACTTTCTATTTGCACATCTGTTTGCTCTTTCTGCGCACAAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACAC > minE/1215001‑1215125
|
ttCACTTTCTATTTGCACATCTGTTTGCTCTTTCTGCGCACAAATGCTGGCAATGGTTTCCGTGCCCttt > 1:1355796/1‑70 (MQ=255)
ttCACTTTCTATTTGCACATCTGTTTGCTCTTTCTGCGCACAAATGCTGGCAATGGTTTCCCTGCCCttt > 1:731684/1‑70 (MQ=255)
ttCACTTTCTATTTGCACATCTGTTTGCTCTTTCTGCGCACAAATGCTGGCAATGGTTTCCCTGCCCttt > 1:382132/1‑70 (MQ=255)
tCACTTTCTATTTGCACATCTGTTTGCTCTTTCTGCGCACAAATGCTGGCAATGGTTTCCCTGCCCtttt < 1:672496/70‑1 (MQ=255)
ttCTATTTGCACATCTGTTTGCTCTTTCTGCGCACAAATGCTGGCAATTGTTTCCCTGCCCTTTTACCTg > 1:754266/1‑70 (MQ=255)
ttCTATTTGCACATCTGTTTGCTCTTTCTGCGCACAAATGCTGGCAATGGTTTCCCTACCCTTTTAc > 1:1778664/1‑67 (MQ=255)
tCTATTTGCACATCTGTTTGCTCTTTCTGCGCACAAATGCTGGCAATGGTTTCCCTACCCTTTTACCTGc < 1:946147/70‑1 (MQ=255)
cTATTTGCACATCTGTTTGCTCTTTCTGCGCACAAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCa < 1:273887/70‑1 (MQ=255)
cTATTTGCACATCTGTTTGCTCTTTCTGCGCACAAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCa > 1:2082165/1‑70 (MQ=255)
tttGCACATCTGTTTGCTCTTTCTGCGCACAAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCAAAcc < 1:1569971/71‑1 (MQ=255)
tttGCACATCTGTTTGCTCTTTCTGCGCACAAATGCTGGCAATGGTTTCCCTACCCTTTTACCTGCaa < 1:1192610/68‑1 (MQ=255)
tttGCACATCTGTTTGCTCTTTCTGCGCACAAATGCTGGCAATGGTTTCCC‑‑CCCTTTTACCTGCAAACCg < 1:1038620/70‑1 (MQ=255)
gCACATCTGTTTGCTCTTTCTGCGCACAAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCAAACCGt > 1:1839675/1‑70 (MQ=255)
cacaTCTGTTTGCTCTTTCTGCGCACAAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCAAACCGTGc < 1:18192/71‑1 (MQ=255)
acaTCTGTTTGCTCTTTCTGCGCACAAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCAAACCGTGCt < 1:2099862/71‑1 (MQ=255)
cTGTTTGCTCTTTCTGCGCACAAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCAAACCGTGCTCggg > 1:473142/1‑71 (MQ=255)
cTGTTTGCTCTTTCTGCGCACAAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCAAACCGTGCTCg > 1:272987/1‑69 (MQ=255)
tGTTTGCTCTTTCTGCGCACAAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCAAACCGTGCTCGGGc > 1:1516983/1‑71 (MQ=255)
tttGCTCTTTCTGCGCACAAATGCTGGCAATGGTTTCCCTGc < 1:306162/42‑1 (MQ=255)
tGCTCTTTCTGCGCACAAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCAAACCGTGCTCGGGCGTa > 1:958363/1‑70 (MQ=255)
tctTTCTGCGCACAAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCAAACCGTGCTc < 1:1850657/60‑1 (MQ=255)
ctTTCTGCGCACAAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTg > 1:481792/1‑69 (MQ=255)
tttCTGCGCACAAATGCTGGCAATGGTTTCCCTGCCCttt < 1:536175/40‑1 (MQ=255)
tttCTGCGCACAAATGCTGCCAATGGTTTCCCTGCCCttt < 1:1479827/40‑1 (MQ=255)
ttCTGCGCACAAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGt > 1:1056522/1‑71 (MQ=255)
tCTGCGCACAAATGCTGGCAATGGTTTCCCTGCCCTTTTAc > 1:383264/1‑41 (MQ=255)
tCTGCGCACAAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCaa > 1:169568/1‑47 (MQ=255)
tGCGCACAAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCg > 1:1070564/1‑70 (MQ=255)
cgcACAAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGa < 1:1517559/69‑1 (MQ=255)
cgcACAAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGa < 1:26413/69‑1 (MQ=255)
cgcACAAATGCTGGCAATGGTTTCCCTACCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGaa > 1:851798/1‑70 (MQ=255)
cgcACAAATGCTGGCAATGGTTTCCCTACCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGaa < 1:1278763/70‑1 (MQ=255)
acaAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAg > 1:439276/1‑71 (MQ=255)
caAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCAAACCGTGc < 1:449546/46‑1 (MQ=255)
caAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAgg > 1:987132/1‑71 (MQ=255)
caAATGCTGGCAATGGTTTCCCTACCCTTTTACCTGCAAACCGTGCTCGGGCGTAGt < 1:1008783/57‑1 (MQ=255)
aaTGCTGGCAATGGTTTCCCTACGCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGtt < 1:1787865/71‑1 (MQ=255)
gCAATGGTTTCCCTACCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCt < 1:528337/71‑1 (MQ=255)
aaTGGTTTCCCTGCCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGa > 1:453560/1‑71 (MQ=255)
aTGGTTTCCCTGCCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGac < 1:92987/71‑1 (MQ=255)
ggTTTCCCTGCCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGacac > 1:620877/1‑71 (MQ=255)
ggTTTCCCTGCCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGacac > 1:83164/1‑71 (MQ=255)
ggTTTCCCTACCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGacac > 1:1290405/1‑71 (MQ=255)
gTTTCCCTGCCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTAc > 1:694297/1‑61 (MQ=255)
tGCCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAAc > 1:794580/1‑46 (MQ=255)
|
TTCACTTTCTATTTGCACATCTGTTTGCTCTTTCTGCGCACAAATGCTGGCAATGGTTTCCCTGCCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACAC > minE/1215001‑1215125
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
