Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I3 R1 176 0.0 1562086 58.2% 909134 66.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,591,415 G→T K203N (AAG→AAT)  yfeS → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,591,4150GT100.0% 203.1 / NA 61K203N (AAG→AAT) yfeSconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (0/61);  total (0/61)

AAAGATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAGG  >  minE/1591412‑1591482
   |                                                                   
aaaTATGTGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATTGGGTGACGGTCAgg  <  1:1094533/71‑1 (MQ=255)
aaaTATGGGACTCAATTCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:1284343/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCggg  <  1:962364/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:607823/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:720292/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:682068/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:675748/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:674353/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:669089/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:667650/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:657302/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:78445/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:590482/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:589489/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:492221/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:477145/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:473512/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:728931/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:731669/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:379086/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:802670/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:837182/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:842623/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:872129/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:886781/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:927378/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:933270/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:957926/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:975724/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:995886/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:999534/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:1450344/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:1056781/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:1131810/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:1150167/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:1162384/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:1180706/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:1207159/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:122702/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:1261552/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:1302936/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:136385/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:1368591/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:1419151/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:1424018/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:417521/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:1458209/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:1503550/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:1513222/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:1519497/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:1555020/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:159485/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:176516/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:198848/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:203376/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:236563/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:291693/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:316881/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAgg  <  1:1040020/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGGCAgg  <  1:77684/71‑1 (MQ=255)
aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGGCAgg  <  1:2697/71‑1 (MQ=255)
   |                                                                   
AAAGATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAGG  >  minE/1591412‑1591482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A