Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I1 R2
|
146 |
74.7 |
3299319 |
84.8% |
2797822 |
66.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,340,015 |
T→C |
K64K (AAA→AAG) |
bipA ← |
GTP‑binding protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,340,015 | 0 | T | C | 100.0%
| 42.0
/ NA
| 13 | K64K (AAA→AAG) | bipA | GTP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/0); total (13/0) |
TTCACCACCGAAGTCGGCGTGCCCCGGGGTATCAACGATGTTGATACGGTAATCATTCCATTTGATAGCGGTGTT > minE/2339955‑2340029
|
ttCACCACCGAAGTCGGCGTGCCCCGGGGTATCAACGATGTTGATACGGTAATCATTCCAttt < 1:2305673/63‑1 (MQ=255)
ttCACCACCGAAGTCGGCGTGCCCCGGGGTATCAACGATGTTGATACGGTAATCATTCCAttt < 1:657896/63‑1 (MQ=255)
ttCACCACCGAAGTCGGCGTGCCCCGGGGTATCAACGATGTTGATACGGTAATCATTCCAttt < 1:433836/63‑1 (MQ=255)
ttCACCACCGAAGTCGGCGTGCCCCGGGGTATCAACGATGTTGATACGGTAATCATTCCAttt < 1:421409/63‑1 (MQ=255)
ttCACCACCGAAGTCGGCGTGCCCCGGGGTATCAACGATGTTGATACGGTAATCATTCCAttt < 1:410187/63‑1 (MQ=255)
ttCACCACCGAAGTCGGCGTGCCCCGGGGTATCAACGATGTTGATACGGTAATCATTCCAttt < 1:2759740/63‑1 (MQ=255)
ttCACCACCGAAGTCGGCGTGCCCCGGGGTATCAACGATGTTGATACGGTAATCATTCCAttt < 1:2691364/63‑1 (MQ=255)
ttCACCACCGAAGTCGGCGTGCCCCGGGGTATCAACGATGTTGATACGGTAATCATTCCAttt < 1:2574183/63‑1 (MQ=255)
ttCACCACCGAAGTCGGCGTGCCCCGGGGTATCAACGATGTTGATACGGTAATCATTCCAttt < 1:2392162/63‑1 (MQ=255)
ttCACCACCGAAGTCGGCGTGCCCCGGGGTATCAACGATGTTGATACGGTAATCATTCCAttt < 1:11769/63‑1 (MQ=255)
ttCACCACCGAAGTCGGCGTGCCCCGGGGTATCAACGATGTTGATACGGTAATCATTCCAttt < 1:2260285/63‑1 (MQ=255)
ttCACCACCGAAGTCGGCGTGCCCCGGGGTATCAACGATGTTGATACGGTAATCATTCCAttt < 1:2096521/63‑1 (MQ=255)
ttCACCACCGAAGTCGGCGTGCCCCGGGGTATCAACGATGTTGATACGGTAATCATTCCAttt < 1:205885/63‑1 (MQ=255)
ttCACCACCGAAGTCGGCGTGCCCCGGGGTATCAACGATGTTGATACGGTAATCATTCCAttt < 1:1999999/63‑1 (MQ=255)
ttCACCACCGAAGTCGGCGTGCCCCGGGGTATCAACGATGTTGATACGGTAATCATTCCAttt < 1:1521137/63‑1 (MQ=255)
ccgccGAAGTCGGCGTGACCGGGGGTGTCGACGATGTTGATGTTGTAGCCGTTCCACTTGATCGCGgtgt > 1:1994352/4‑70 (MQ=255)
ccgccGAAGTCGGCGTGACCGGGGGTGTCGACGATGTTGATGTTGTAGCCGTTCCACTTGATCGCGgtgt > 1:2405179/4‑70 (MQ=255)
ccgccGAAGTCGGCGTGACCGGGGGTGTCGACGATGTTGATGTTGTAGCCGTTCCACTTGATCGCGgtgt > 1:2500610/4‑70 (MQ=255)
ccgccGAAGTCGGCGTGACCGGGGGTGTCGACGATGTTGATGTTGTAGCCGTTCCACTTGATCGCGgtgt > 1:1861797/4‑70 (MQ=255)
ccgccGAAGTCGGCGTGACCGGGGGTGTCGACGATGTTGATGTTGTAGCCGTTCCACTTGATCGCGgtgt > 1:1822732/4‑70 (MQ=255)
ccgccGAAGTCGGCGTGACCGGGGGTGTCGACGATGTTGATGTTGTAGCCGTTCCACTTGATCGCGgtgt > 1:1748632/4‑70 (MQ=255)
ccgccGAAGTCGGCGTGACCGGGGGTGTCGACGATGTTGATGTTGTAGCCGTTCCACTTGATCGCGgtgt > 1:3288753/4‑70 (MQ=255)
ccgccGAAGTCGGCGTGACCGGGGGTGTCGACGATGTTGATGTTGTAGCCGTTCCACTTGATCGCGgtgt > 1:408549/4‑70 (MQ=255)
ccgccGAAGTCGGCGTGACCGGGGGTGTCGACGATGTTGATGTTGTAGCCGTTCCACTTGATCGCGgtgt > 1:1742886/4‑70 (MQ=255)
ccgccGAAGTCGGCGTGACCGGGGGTGTCGACGATGTTGATGTTGTAGCCGTTCCACTTGATCGCGgtgt > 1:1454184/4‑70 (MQ=255)
ccgccGAAGTCGGCGTGACCGGGGGTGTCGACGATGTTGATGTTGTAGCCGTTCCACTTGATCGCGGTGtt > 1:1980951/4‑71 (MQ=255)
ccgccGAAGTCGGCGTGACCGGGGGTGTCGACGATGTTGATGTTGTAGCCGTTCCACTTGATCGCGGTGtt > 1:3046224/4‑71 (MQ=255)
ccgccGAAGTCGGCGTGACCGGGGGTGTCGACGATGTTGATGTTGTAGCCGTTCCACTTGATCGCGGTGtt > 1:1584411/4‑71 (MQ=255)
|
TTCACCACCGAAGTCGGCGTGCCCCGGGGTATCAACGATGTTGATACGGTAATCATTCCATTTGATAGCGGTGTT > minE/2339955‑2340029
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
