Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R2
|
422 |
46.8 |
3726540 |
94.7% |
3529033 |
61.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
494,006 |
T→A |
9.1% |
D126E (GAT→GAA) |
recR → |
gap repair protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 494,006 | 0 | T | A | 9.1%
| 182.9
/ 9.9
| 66 | D126E (GAT→GAA) | recR | gap repair protein |
| Reads supporting (aligned to +/- strand): ref base T (33/27); new base A (4/2); total (37/29) |
| Fisher's exact test for biased strand distribution p-value = 6.88e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01 |
GTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGA > W3110S.gb/493939‑494074
|
gTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCg < 1:1449359/70‑1 (MQ=255)
gggCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTgg > 1:3318594/1‑70 (MQ=255)
gggCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTgg > 1:3257463/1‑70 (MQ=255)
tGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGa > 1:1786799/1‑70 (MQ=255)
tGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGa > 1:127174/1‑70 (MQ=255)
tGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGa > 1:351664/1‑70 (MQ=255)
tGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGa > 1:1452116/1‑70 (MQ=255)
tGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGAACGTCTGGAACAGCGTCTGGCAGAGGa > 1:3248187/1‑70 (MQ=255)
tGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGAACGTCTGGAACAGCGTCTGGCAGAGGa > 1:1768366/1‑70 (MQ=255)
gTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGa > 1:1211589/1‑51 (MQ=255)
gTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGa > 1:1356677/1‑51 (MQ=255)
gTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGa > 1:850439/1‑51 (MQ=255)
tCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTgg < 1:397295/61‑1 (MQ=255)
aCCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGaaaaaa > 1:1363854/1‑71 (MQ=255)
aCCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGaaaaaa > 1:3328931/1‑71 (MQ=255)
aCCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGaaaaaa > 1:2469520/1‑71 (MQ=255)
aCCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGaaaaa < 1:3711806/70‑1 (MQ=255)
gACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTg < 1:28070/70‑1 (MQ=255)
gACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTg < 1:1239258/70‑1 (MQ=255)
gACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTg < 1:2574618/70‑1 (MQ=255)
gACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGa < 1:2390814/71‑1 (MQ=255)
gACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGa < 1:759997/71‑1 (MQ=255)
tCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATc > 1:807495/1‑71 (MQ=255)
tCGGTCCGGATGATATCGGGCTTGATCGTCTCGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATc > 1:1402260/1‑71 (MQ=255)
ggTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGAt > 1:348357/1‑68 (MQ=255)
ggTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGAt > 1:905415/1‑68 (MQ=255)
ggTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGAt > 1:318438/1‑68 (MQ=255)
ggTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGAt > 1:3171685/1‑68 (MQ=255)
ggTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGAt > 1:284412/1‑68 (MQ=255)
ggTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGAt > 1:3075369/1‑68 (MQ=255)
ggTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCt > 1:1091643/1‑71 (MQ=255)
gTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATcc < 1:2917863/69‑1 (MQ=255)
gTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATcc < 1:2508339/69‑1 (MQ=255)
ccGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTc > 1:2515860/1‑69 (MQ=255)
atatCGGGCTTGAACGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAAc > 1:1164905/1‑71 (MQ=255)
atatCGGGCTTGAACGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAAc > 1:376000/1‑71 (MQ=255)
atCGGGCTTGATCGTCTGGAACAGCGTCTGGGAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAAccc > 1:1019325/1‑71 (MQ=255)
atCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAAccc > 1:728725/1‑71 (MQ=255)
atCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAAcc > 1:276338/1‑70 (MQ=255)
tCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGa < 1:1754248/37‑1 (MQ=255)
gggCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCAc < 1:973993/71‑1 (MQ=255)
gggCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCAc > 1:744434/1‑71 (MQ=255)
gggCTTGATCGTCTGGAACAGCGTCGCGCAGAGGAAAAAAt > 1:3280744/1‑41 (MQ=38)
ggCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTg < 1:3132066/50‑1 (MQ=255)
ggCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTg < 1:2465826/50‑1 (MQ=255)
cTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGAt > 1:87603/1‑50 (MQ=255)
cTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGAt > 1:1544415/1‑50 (MQ=255)
cTTGAACGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTg < 1:3199491/48‑1 (MQ=255)
cTTGAACGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTg < 1:830052/48‑1 (MQ=255)
ttGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACg > 1:107451/1‑68 (MQ=255)
ttGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGtt > 1:3277554/1‑71 (MQ=255)
ttGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGtt > 1:846040/1‑71 (MQ=255)
ttGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGtt > 1:2303295/1‑71 (MQ=255)
gATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTg < 1:2179485/70‑1 (MQ=255)
gATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTg < 1:832681/70‑1 (MQ=255)
gATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTg < 1:87497/70‑1 (MQ=255)
gATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTg < 1:1133823/70‑1 (MQ=255)
aTCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTg < 1:600928/44‑1 (MQ=255)
aTCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGa < 1:405382/45‑1 (MQ=255)
aTCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGa < 1:2195335/45‑1 (MQ=255)
aTCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGa < 1:1799248/45‑1 (MQ=255)
aTCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGa < 1:400906/45‑1 (MQ=255)
aTCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGa < 1:3578219/70‑1 (MQ=255)
aTCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGa < 1:152852/70‑1 (MQ=255)
aTCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGa < 1:3227210/70‑1 (MQ=255)
aTCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGa < 1:3414781/70‑1 (MQ=255)
|
GTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGA > W3110S.gb/493939‑494074
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
