Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R2
|
230 |
93.6 |
4701257 |
96.3% |
4527310 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,837,204 |
T→C |
26.1% |
E39G (GAA→GGA) |
yjeK ← |
predicted lysine aminomutase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,837,204 | 0 | T | C | 26.1%
| 78.5
/ 31.1
| 51 | E39G (GAA→GGA) | yjeK | predicted lysine aminomutase |
| Reads supporting (aligned to +/- strand): ref base T (13/24); new base C (8/5); total (22/29) |
| Fisher's exact test for biased strand distribution p-value = 1.16e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 |
CGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGCAAGTTGCGTT > minE/2837135‑2837272
|
cgatcgatAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTtc > 1:367900/1‑71 (MQ=255)
atAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTCCCTCcgc < 1:1861748/71‑1 (MQ=255)
tAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTCCCTCcgcg > 1:2488574/1‑71 (MQ=255)
aaTGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTc < 1:1087215/71‑1 (MQ=255)
aGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCtata > 1:2171334/1‑71 (MQ=255)
cacGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTCCCTCCGCGTCTATATTCAAAAGa > 1:539861/1‑71 (MQ=255)
cGCAGGGCAAAAAGCTTTTTGGCGCTGCGTAAGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGAc > 1:1971145/1‑70 (MQ=255)
gCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACg > 1:3136112/1‑70 (MQ=255)
aGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAg < 1:2837239/71‑1 (MQ=255)
aGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTCCCTCCGCGTCTATATTCAAAAGACGCAg < 1:1750510/71‑1 (MQ=255)
ggCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGAc < 1:3092369/65‑1 (MQ=255)
ggCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAg > 1:574489/1‑69 (MQ=255)
ggCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGa < 1:594269/70‑1 (MQ=255)
gCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGAc > 1:1677525/1‑64 (MQ=255)
gCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTCCCTCCGCGTCTATATTCAAAAGACGCAGAAg > 1:1266071/1‑71 (MQ=255)
cAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTCCCTCCGCGTCTATATTCAAAAGACGCAGAAGt > 1:4517700/1‑71 (MQ=255)
aaaaGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGtt > 1:1348110/1‑70 (MQ=255)
aaaaGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGtt > 1:905609/1‑70 (MQ=255)
aaaGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTtca < 1:726527/71‑1 (MQ=255)
aGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCaaaa > 1:1532222/1‑55 (MQ=255)
tttttGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTtca < 1:3394777/66‑1 (MQ=255)
tttttGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTtca < 1:2567828/66‑1 (MQ=255)
tttttGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTtc < 1:2575059/65‑1 (MQ=255)
tttttGGCGCTGCGTCCGGCTAACAGTTTTACCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAg > 1:1574120/1‑70 (MQ=255)
ttttGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGa > 1:4186769/1‑71 (MQ=255)
ttttGGCGCTGCGTCCGGCTAACAGTTTTCCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGa > 1:972868/1‑71 (MQ=255)
tttGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAgg < 1:611251/69‑1 (MQ=255)
tttGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGAt > 1:2103004/1‑71 (MQ=255)
ttGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGAt < 1:1748698/70‑1 (MQ=255)
ttGGCGCTGCGTCAGGCTAACAGTTTTCCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGa < 1:3422080/69‑1 (MQ=255)
ggCGCTGCGTCCGGCTAACAGTTTTCCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAggt > 1:2974499/1‑66 (MQ=255)
gcTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTca > 1:708063/1‑71 (MQ=255)
cTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTcac < 1:1309469/71‑1 (MQ=255)
cTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTcac < 1:2286513/71‑1 (MQ=255)
tGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCaaaa < 1:2789719/42‑1 (MQ=255)
tGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTcac > 1:2755181/1‑70 (MQ=255)
tGCGTCCGGCTAACAGTTTTCCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTcac > 1:3805300/1‑70 (MQ=255)
cGTCCGGCTAACAGTTTTCCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCacaac < 1:3251887/71‑1 (MQ=255)
tcaggCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACAt < 1:4224415/68‑1 (MQ=255)
tCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCacaaca > 1:1274591/1‑70 (MQ=255)
tCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACAt < 1:4343032/71‑1 (MQ=255)
cGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCg < 1:3644892/71‑1 (MQ=255)
gCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGt < 1:3460306/45‑1 (MQ=255)
gCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTcac < 1:4547469/62‑1 (MQ=255)
gCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGc < 1:186624/71‑1 (MQ=255)
gCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGc < 1:140642/71‑1 (MQ=255)
cTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGc < 1:143864/70‑1 (MQ=255)
aaCAGTTTTCCCTCCGCGTCTATATTCAAAAGACGCAg < 1:732662/38‑1 (MQ=255)
aCAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGCaa < 1:1016060/69‑1 (MQ=255)
aCAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGCaa < 1:2539962/69‑1 (MQ=255)
aCAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGCaa < 1:2545455/69‑1 (MQ=255)
aGTTTTCCCTCCGCGTCTATATTCAAAAGACGCAGAAGt > 1:1916647/1‑39 (MQ=255)
tttttCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGCAAGTTGc > 1:3748100/1‑70 (MQ=255)
tttcCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTca > 1:4165931/1‑52 (MQ=255)
tttcCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGCAAGTTGCGt > 1:289099/1‑71 (MQ=255)
tttCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGCAAGTTGCGt > 1:536022/1‑70 (MQ=255)
ttCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGCAAGTTGCGtt < 1:791879/70‑1 (MQ=255)
|
CGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGCAAGTTGCGTT > minE/2837135‑2837272
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
