Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R2
|
232 |
76.3 |
4135488 |
90.9% |
3759158 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,384,474 |
G→A |
13.3% |
L363L (CTG→TTG) |
metE ← |
5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,384,474 | 0 | G | A | 13.3%
| 251.5
/ 25.3
| 90 | L363L (CTG→TTG) | metE | 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (40/38); new base A (5/7); total (45/45) |
| Fisher's exact test for biased strand distribution p-value = 7.58e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 |
GGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTG > minE/2384406‑2384542
|
ggTAGAGTGACGACGTGCCTGAATAGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAgcg < 1:924524/71‑1 (MQ=255)
tAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCa > 1:992301/1‑71 (MQ=255)
gagTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAg < 1:3463208/65‑1 (MQ=255)
gagTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAg < 1:3493102/65‑1 (MQ=255)
gagTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAg < 1:36010/65‑1 (MQ=255)
gagTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAg < 1:1672229/65‑1 (MQ=255)
gagTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAg < 1:1532096/65‑1 (MQ=255)
gacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCgcagc < 1:1030434/71‑1 (MQ=255)
gacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCgcagc < 1:1573472/71‑1 (MQ=255)
gacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCgcagc < 1:1553814/71‑1 (MQ=255)
gacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCgcagc < 1:144022/71‑1 (MQ=255)
gacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCgcag > 1:580502/1‑70 (MQ=255)
gacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGAgcag > 1:744520/1‑70 (MQ=255)
acgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCgcagc < 1:3438990/70‑1 (MQ=255)
ccTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGc > 1:231762/1‑70 (MQ=255)
tGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAg > 1:2679200/1‑71 (MQ=255)
tGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAg > 1:211605/1‑71 (MQ=255)
aaTCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGt > 1:1793144/1‑70 (MQ=255)
ggggCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCAt > 1:521600/1‑49 (MQ=255)
ggggCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCAt > 1:2143992/1‑49 (MQ=255)
cTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGGCAGTTCATGGCAttt > 1:3048397/1‑71 (MQ=255)
cTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCAttt > 1:3414020/1‑71 (MQ=255)
cTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCAttt > 1:3963145/1‑71 (MQ=255)
ccACTCTGACAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCAtttt > 1:2709073/1‑70 (MQ=255)
cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:1189413/71‑1 (MQ=255)
cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:2357012/71‑1 (MQ=255)
cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:3219195/71‑1 (MQ=255)
cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:2450233/71‑1 (MQ=255)
cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:3489890/71‑1 (MQ=255)
cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:3406489/71‑1 (MQ=255)
cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:3199369/71‑1 (MQ=255)
cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:307096/71‑1 (MQ=255)
cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:691546/71‑1 (MQ=255)
cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg > 1:1663265/1‑71 (MQ=255)
cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:2969347/71‑1 (MQ=255)
cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:4081980/71‑1 (MQ=255)
cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg < 1:1266250/71‑1 (MQ=255)
cACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg > 1:1458930/1‑71 (MQ=255)
cACTCTGCCAGAGCTGCCGTGTAACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg > 1:1128089/1‑71 (MQ=255)
ctGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAgg > 1:1599981/1‑71 (MQ=255)
tGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCAttttt > 1:549880/1‑65 (MQ=255)
tGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCAttttt > 1:282847/1‑65 (MQ=255)
gagCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAg > 1:3786761/1‑71 (MQ=255)
gagCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAg > 1:2436248/1‑71 (MQ=255)
gagCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAg > 1:3858875/1‑71 (MQ=255)
gagCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAg > 1:1880534/1‑71 (MQ=255)
gagCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAg > 1:472278/1‑71 (MQ=255)
agCTGCCGTGTCACCACTGTTCAGCGCATCGCgcagc < 1:4071566/37‑1 (MQ=255)
gCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTg > 1:2982017/1‑59 (MQ=255)
gCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCAt > 1:56710/1‑46 (MQ=255)
gCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCaaa > 1:1520747/1‑71 (MQ=255)
cGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAAcc < 1:291584/71‑1 (MQ=255)
gtgtCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCaaa > 1:3581046/1‑68 (MQ=255)
gtgtCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAAc > 1:1334222/1‑69 (MQ=255)
gtgtCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAAc > 1:2318466/1‑69 (MQ=255)
gtgtCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAAc > 1:3576565/1‑69 (MQ=255)
gtgtCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAAc > 1:259854/1‑69 (MQ=255)
gtatCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCa < 1:3006751/68‑1 (MQ=255)
gtatCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCa < 1:679723/68‑1 (MQ=255)
gtatCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCa < 1:2054522/68‑1 (MQ=255)
gtatCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCa < 1:1143566/68‑1 (MQ=255)
gtatCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCa < 1:354384/68‑1 (MQ=255)
gtatCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCa < 1:2547815/68‑1 (MQ=255)
gtatCACCACTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCa < 1:2837048/68‑1 (MQ=255)
tgtCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCAttt < 1:3566495/49‑1 (MQ=255)
tgtCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCAttt < 1:3202546/49‑1 (MQ=255)
tCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCt < 1:247978/71‑1 (MQ=255)
tCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCt < 1:3268882/71‑1 (MQ=255)
tCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCt < 1:3208637/71‑1 (MQ=255)
accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt > 1:2531894/1‑71 (MQ=255)
accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt > 1:116741/1‑71 (MQ=255)
accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt < 1:2972727/71‑1 (MQ=255)
accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt > 1:1407526/1‑71 (MQ=255)
accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt > 1:503024/1‑71 (MQ=255)
accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtt > 1:2934076/1‑70 (MQ=255)
accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtt < 1:777133/70‑1 (MQ=255)
accacTGTTCAGCGCATCGCGCAGCAGTCCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt < 1:513235/71‑1 (MQ=255)
ccacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtttt < 1:3164271/71‑1 (MQ=255)
ccacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtttt < 1:2835817/71‑1 (MQ=255)
ccacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtttt < 1:1608458/71‑1 (MQ=255)
ccacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtttt < 1:917543/71‑1 (MQ=255)
cacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTGATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt < 1:1661228/69‑1 (MQ=255)
cTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGGaa < 1:1769867/58‑1 (MQ=255)
cTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCaa < 1:3650997/58‑1 (MQ=255)
cTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCaa < 1:552048/58‑1 (MQ=255)
cTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCaa < 1:570389/58‑1 (MQ=255)
gTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCa > 1:3826583/1‑60 (MQ=255)
gTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCAttt > 1:2579579/1‑71 (MQ=255)
gTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACtt > 1:3077669/1‑71 (MQ=255)
ttCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGc > 1:3507569/1‑53 (MQ=255)
ttCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTc > 1:3027212/1‑71 (MQ=255)
cAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTg > 1:1541899/1‑71 (MQ=255)
cAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTg > 1:2418600/1‑71 (MQ=255)
cAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTg > 1:1091904/1‑71 (MQ=255)
aGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTg < 1:1219284/70‑1 (MQ=255)
aGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTg < 1:3886369/70‑1 (MQ=255)
|
GGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTG > minE/2384406‑2384542
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
