Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F21 I0 R1
|
57 |
0.0 |
661283 |
90.4% |
597799 |
68.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,666,947:1 |
+C |
100% |
coding (1065/2706 nt) |
malT ← |
DNA‑binding transcriptional activator, maltotriose‑ATP‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,666,947 | 1 | . | C | 100.0%
| 43.5
/ NA
| 13 | G355G (GGA→GGG) | malT | DNA‑binding transcriptional activator, maltotriose‑ATP‑binding |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (0/13); total (0/13) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GGAAAT‑CCCTGGGCCATCCAGCTTTCTGCGGCGGCACGGTGGATTTCCGGCAGCTCCGCCGCCAGTTCCC > minE/2666942‑2667011
|
ggAAATCCCCTGGGCCATCCAGCTTTCTGCGGCGGCACGGTGGATTTCCGGCAGCTCCGCCGCCAGTTccc < 1:17160/71‑1 (MQ=255)
ggAAATCCCCTGGGCCATCCAGCTTTCTGCGGCGGCACGGTGGATTTCCGGCAGCTCCGCCGCCAGTTccc < 1:179815/71‑1 (MQ=255)
ggAAATCCCCTGGGCCATCCAGCTTTCTGCGGCGGCACGGTGGATTTCCGGCAGCTCCGCCGCCAGTTccc < 1:227103/71‑1 (MQ=255)
ggAAATCCCCTGGGCCATCCAGCTTTCTGCGGCGGCACGGTGGATTTCCGGCAGCTCCGCCGCCAGTTccc < 1:333433/71‑1 (MQ=255)
ggAAATCCCCTGGGCCATCCAGCTTTCTGCGGCGGCACGGTGGATTTCCGGCAGCTCCGCCGCCAGTTccc < 1:36844/71‑1 (MQ=255)
ggAAATCCCCTGGGCCATCCAGCTTTCTGCGGCGGCACGGTGGATTTCCGGCAGCTCCGCCGCCAGTTccc < 1:37469/71‑1 (MQ=255)
ggAAATCCCCTGGGCCATCCAGCTTTCTGCGGCGGCACGGTGGATTTCCGGCAGCTCCGCCGCCAGTTccc < 1:394413/71‑1 (MQ=255)
ggAAATCCCCTGGGCCATCCAGCTTTCTGCGGCGGCACGGTGGATTTCCGGCAGCTCCGCCGCCAGTTccc < 1:492511/71‑1 (MQ=255)
ggAAATCCCCTGGGCCATCCAGCTTTCTGCGGCGGCACGGTGGATTTCCGGCAGCTCCGCCGCCAGTTccc < 1:539163/71‑1 (MQ=255)
ggAAATCCCCTGGGCCATCCAGCTTTCTGCGGCGGCACGGTGGATTTCCGGCAGCTCCGCCGCCAGTTccc < 1:81478/71‑1 (MQ=255)
gAAATCCCCTGGGCCATCCAGCTTTCTGCGGCGGCACGGTGGATTTCCGGCAGCTCCGCCGCCAGTTccc < 1:354428/70‑1 (MQ=255)
gAAATCCCCTGGGCCATCCAGCTTTCTGCGGCGGCACGGTGGATTTCCGGCAGCTCCGCCGCCAGTTccc < 1:381377/70‑1 (MQ=255)
gAAATCCCCTGGGCCATCCAGCTTTCTGCGGCGGCACGGTGGATTTCCGGCAGCTCCGCCGCCAGTTccc < 1:99669/70‑1 (MQ=255)
|
GGAAAT‑CCCTGGGCCATCCAGCTTTCTGCGGCGGCACGGTGGATTTCCGGCAGCTCCGCCGCCAGTTCCC > minE/2666942‑2667011
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
