Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I3 R2 68 0.0 1607493 93.4% 1501398 66.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,862,014 +T coding (1033/1077 nt) ydjL ← predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,862,0101.T100.0% 640.2 / NA 180T346N (ACC→AAC) ydjLpredicted oxidoreductase, Zn‑dependent and NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (0/180);  total (0/180)

Only 100 of 180 total aligned reads displayed.

GATCACTTTGATTGCGG‑TTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAC  >  W3110S.gb/1861994‑1862061
                 |                                                   
gATCACTTTGATTGCGGTTTTTATCTACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:777626/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGacac  <  1:151005/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGacac  <  1:152988/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:461056/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:5750/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:555859/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:553044/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:530685/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:526191/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:521718/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:516940/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:512275/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:476079/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:617599/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:438758/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:429806/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:385779/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:377724/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:357398/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:350359/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:343629/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:331150/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:313389/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:311226/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:264554/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:80173/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:977812/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:930933/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:892909/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:89079/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:885429/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:872701/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:86202/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:858261/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:854605/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:837535/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:819694/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:589702/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:791220/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:78838/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:760015/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:718745/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:692683/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:679617/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:650709/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:641405/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:640495/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:624608/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:304911/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1116916/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1438554/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1422414/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1410347/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1404023/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:235156/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:13618/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:134560/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1326757/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1291370/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:127146/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1101726/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1231630/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1122634/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:12131/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1207841/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1193850/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1182777/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1180322/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1168293/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1160518/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1084557/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1029363/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:233426/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:227250/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:213818/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:201681/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:169846/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1602946/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1594859/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1586346/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1580383/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:156543/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1096665/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1522661/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1516718/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1496567/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1496352/69‑1 (MQ=255)
gATCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1479063/69‑1 (MQ=255)
 aTCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1390805/68‑1 (MQ=255)
 aTCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:960772/68‑1 (MQ=255)
 aTCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1138819/68‑1 (MQ=255)
 aTCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:746140/68‑1 (MQ=255)
 aTCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:700968/68‑1 (MQ=255)
 aTCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1551091/68‑1 (MQ=255)
 aTCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:407956/68‑1 (MQ=255)
 aTCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1020047/68‑1 (MQ=255)
 aTCACTTTGATTGCGGTTTTTATCGACCATAGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1037171/68‑1 (MQ=255)
 aTCACTTTGACTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:1444691/68‑1 (MQ=255)
  tCACTTTGATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:105227/67‑1 (MQ=255)
         gATTGCGGTTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAc  <  1:225959/60‑1 (MQ=255)
                 |                                                   
GATCACTTTGATTGCGG‑TTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATAC  >  W3110S.gb/1861994‑1862061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A