Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I3 R2 68 0.0 1607493 93.4% 1501398 66.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,342,658 T→G Y478S (TAC→TCC)  gyrA ← DNA gyrase (type II topoisomerase), subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,342,6580TG100.0% 247.3 / NA 67Y478S (TAC→TCC) gyrADNA gyrase (type II topoisomerase), subunit A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (67/0);  total (67/0)

CCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTAT  >  W3110S.gb/2342593‑2342660
                                                                 |  
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:385588/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:648298/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:623018/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:583295/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:497791/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:47388/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:464322/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:445022/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:4358/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:653384/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:365016/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:361088/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:347693/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:330328/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:313594/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:293381/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:287513/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:859068/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:996667/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:964611/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:944721/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:939975/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:929179/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:910683/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:870928/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:860041/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:263849/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:842261/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:841660/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:831686/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:805058/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:75157/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:700000/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:69670/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1275314/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1386185/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1368818/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1366695/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1319101/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1318123/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1312085/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1306056/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1291713/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:14098/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1254316/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1218256/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1210044/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1189995/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1175336/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1066761/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1060257/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1006918/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1417455/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1431169/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1455124/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1466641/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1521609/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1543169/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1575909/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:1586617/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:168985/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:172769/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:182973/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:193761/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:214463/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:228265/1‑68 (MQ=255)
ccATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGGAt  >  1:262958/1‑68 (MQ=255)
                                                                 |  
CCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTAT  >  W3110S.gb/2342593‑2342660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A