Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I3 R2 68 0.0 1607493 93.4% 1501398 66.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,445,129 A→G S146S (TCT→TCC)  yfcJ ← predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,445,1290AG100.0% 955.0 / NA 253S146S (TCT→TCC) yfcJpredicted transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/253);  total (0/253)

Only 100 of 253 total aligned reads displayed.

CGAGGGCACCGTAAATCGCCATTCCGTTCCATGACATCACTTTGCCAGAGTGTTTTGGCCCTACGATGC  >  W3110S.gb/2445083‑2445151
                                              |                      
ctaGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTAGCCCAACGATGc  <  1:335036/67‑1 (MQ=255)
cGAGGGCTCCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:534299/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACTATGc  <  1:1278712/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:467063/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:627537/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:609306/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:607971/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:587239/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:572471/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:556747/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:509848/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:495352/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:473787/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:648836/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:424213/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:392999/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:361786/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:350171/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:326520/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:303343/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:282372/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1000769/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:263913/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:2429/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:829925/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:991613/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:947075/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:923379/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:91544/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:907004/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:894996/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:86421/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:85868/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:852244/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:845808/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:835831/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:63351/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:823187/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:820399/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:759672/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:740480/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:73247/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:715333/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:709315/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:679299/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:651065/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:274046/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1171597/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1407563/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:233705/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1358752/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1348981/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1321934/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1296838/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1262286/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1250113/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1238923/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1213431/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1195879/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1359202/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:116013/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1137575/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1124544/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1116495/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:110385/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1090102/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1069507/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1047424/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1032331/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:101637/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1551376/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1599286/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1592128/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1606817/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1563179/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:163564/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:182614/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:192801/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1557078/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1531001/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1500626/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:206252/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:220715/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1425514/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1473242/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1436373/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAACGTTTTGGCCCAACGATGc  <  1:1466966/69‑1 (MQ=255)
cGAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACCTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1389378/69‑1 (MQ=255)
 gAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:254141/68‑1 (MQ=255)
 gAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1079348/68‑1 (MQ=255)
 gAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:385085/68‑1 (MQ=255)
 gAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1561906/68‑1 (MQ=255)
 gAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:806106/68‑1 (MQ=255)
 gAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:772597/68‑1 (MQ=255)
 gAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:438765/68‑1 (MQ=255)
 gAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:52827/68‑1 (MQ=255)
 gAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:1493616/68‑1 (MQ=255)
 gAGGGCACCGTAAATAGCCATTCCGTTCCATGACATCACTTAGCCGGAATGTTTTGGCCCAACGATGc  <  1:1050958/68‑1 (MQ=255)
             aaTAGCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:665886/56‑1 (MQ=255)
               tagCCATTCCGTTCCATGACATCACTTTGCCGGAATGTTTTGGCCCAACGATGc  <  1:969951/52‑1 (MQ=255)
                                              |                      
CGAGGGCACCGTAAATCGCCATTCCGTTCCATGACATCACTTTGCCAGAGTGTTTTGGCCCTACGATGC  >  W3110S.gb/2445083‑2445151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A