Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R1
|
71 |
95.7 |
2922988 |
91.7% |
2680379 |
65.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
710,510 |
C→A |
100% |
C302F (TGC→TTC) |
solA ← |
N‑methyltryptophan oxidase, FAD‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 710,510 | 0 | C | A | 91.3%
| 123.2
/ 7.9
| 46 | C302F (TGC→TTC) | solA | N‑methyltryptophan oxidase, FAD‑binding |
| Reads supporting (aligned to +/- strand): ref base C (0/4); new base A (0/42); total (0/46) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
ATCATAGGTGCAGGCAGCGCCGTACAGGCAGCAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAGGCTTCTGACCCATCACTGGCC > minE/710482‑710572
|
aTCCTAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1199364/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAATAACGGGAAg < 1:1181038/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:368628/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2044047/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2265243/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2458150/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:254457/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2699229/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2709827/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2726037/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2756576/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2856523/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:287108/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2119182/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:40672/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:484292/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:554822/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:60805/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:672305/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:868177/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:916160/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2024708/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1131603/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1984821/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1815991/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1146256/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1224401/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1331711/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1776152/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:144945/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1490563/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1504417/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAAAGGGAAg < 1:4544/70‑1 (MQ=255)
aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACACGGCAATACATTGCGCAAGAACGGGAAg < 1:206380/70‑1 (MQ=255)
tCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1589830/69‑1 (MQ=255)
tCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1665571/69‑1 (MQ=255)
tCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2471052/69‑1 (MQ=255)
cATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1723874/68‑1 (MQ=255)
tgTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2661324/63‑1 (MQ=255)
gTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1714729/63‑1 (MQ=255)
aggcagCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1779520/59‑1 (MQ=255)
cGTACAGGCAGCAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAGGCTTCTGACCCATCACTGGcc < 1:1660417/71‑1 (MQ=255)
cGTACAGGCAGCAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAGGCTTCTGACCCATCACTGGcc < 1:1697483/71‑1 (MQ=255)
cGTACAGGCAGCAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAGGCTTCTGACCCATCACTGGcc < 1:908786/71‑1 (MQ=255)
cGTACAGGCAGCAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAGGCTTCTGACCCATCACTGGcc < 1:1075646/71‑1 (MQ=255)
aCAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:633324/47‑1 (MQ=255)
|
ATCATAGGTGCAGGCAGCGCCGTACAGGCAGCAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAGGCTTCTGACCCATCACTGGCC > minE/710482‑710572
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
