Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R1
|
71 |
95.7 |
2922988 |
91.7% |
2680379 |
65.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,839,654 |
T→A |
100% |
N96Y (AAT→TAC) |
surE ← |
broad specificity 5'(3')‑nucleotidase and polyphosphatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,839,654 | 0 | T | A | 90.2%
| 109.4
/ 5.4
| 41 | N96Y (AAT→TAC) | surE | broad specificity 5'(3')‑nucleotidase and polyphosphatase |
| Reads supporting (aligned to +/- strand): ref base T (4/0); new base A (37/0); total (41/0) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01 |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
ACGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGATTCGGCCCGGCGTTAATTCCGGACACAACAATGTCCGGGCGCGGACGCATCAGAGCATTCACGCCAAGATA > minE/1839598‑1839723
|
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACggac > 1:313088/1‑60 (MQ=255)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:674761/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:2440906/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:707593/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:263682/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:1013374/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:2790844/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:2815567/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:2861496/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:2898645/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:2433079/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:352423/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:369871/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:385486/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:491495/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:667644/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:531685/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:531719/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:1727627/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:1015487/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:1230920/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:832202/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:793995/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:1420748/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:1446500/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:1463794/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:1500446/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:1791579/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:1802972/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:1998827/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:207786/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:732422/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:2139602/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:2166431/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGtatt > 1:2319703/1‑68 (MQ=37)
aCGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGGTACGGACCCGCGta > 1:516430/1‑68 (MQ=38)
aCGGCCTTCCATCGCGGCGGCTACAGTACCGGAATAAATAACATCATCCCCCAGGTACgga > 1:2672474/1‑60 (MQ=255)
ataaataaCATCATCCCCCAGATTCGGCCCGGCGTTAATTCCg > 1:209232/1‑43 (MQ=255)
ataaataaCATCATCCCCCAGATTCGGCCCGGCGTTAATTCCGGACACAACAATGTCCGGGCGCGGACGCa > 1:519465/1‑71 (MQ=255)
ataaataaCATCATCCCCCAGATTCGGCCCGGCGTTAATTCCGGACACAACAATGTCCGGGCGCGGACGCa > 1:292564/1‑71 (MQ=255)
ataaataaCATCATCCCCCAGATTCGGCCCGGCGTTAATTCCGGACACAACAATGTCCGGGCGCGGACGCa > 1:713543/1‑71 (MQ=255)
ttCGGCCCGGCGTTAATTCCGGACACAACAATGTCCGGGCGCGGACGCATCAGAGCATTCACGCCAAGATa < 1:2511470/71‑1 (MQ=255)
ttCGGCCCGGCGTTAATTCCGGACACAACAATGTCCGGGCGCGGACGCATCAGAGCATTCACGCCAAGATa < 1:131335/71‑1 (MQ=255)
ttCGGCCCGGCGTTAATTCCGGACACAACAATGTCCGGGCGCGGACGCATCAGAGCATTCACGCCAAGATa < 1:987176/71‑1 (MQ=255)
tCGGCCCGGCGTTAATTCCGGACACAACAATGTCCGGGCGCGGACGCATCAGAGCATTCACGCCAAGATa < 1:1278385/70‑1 (MQ=255)
|
ACGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGATTCGGCCCGGCGTTAATTCCGGACACAACAATGTCCGGGCGCGGACGCATCAGAGCATTCACGCCAAGATA > minE/1839598‑1839723
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
