Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
265,816 |
C→T |
41.0% |
A263A (GCG→GCA) |
yajO ← |
predicted oxidoreductase, NAD(P)‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 265,816 | 0 | C | T | 41.0%
| 30.3
/ 49.9
| 73 | A263A (GCG→GCA) | yajO | predicted oxidoreductase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (12/31); new base T (15/15); total (27/46) |
| Fisher's exact test for biased strand distribution p-value = 8.40e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-02 |
ATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTT > minE/265754‑265883
|
aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:1334657/68‑1 (MQ=255)
aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:154311/68‑1 (MQ=255)
aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:2651486/68‑1 (MQ=255)
aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:1633965/68‑1 (MQ=255)
aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:1442786/68‑1 (MQ=255)
aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:1384770/68‑1 (MQ=255)
aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:1365941/68‑1 (MQ=255)
aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:2475554/68‑1 (MQ=255)
aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:407491/68‑1 (MQ=255)
aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:2173716/68‑1 (MQ=255)
aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:486675/68‑1 (MQ=255)
aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:2136436/68‑1 (MQ=255)
aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:1124042/68‑1 (MQ=255)
aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:750959/68‑1 (MQ=255)
aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:187981/68‑1 (MQ=255)
aaTCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAg < 1:2857149/69‑1 (MQ=255)
aaTCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAg < 1:1580645/69‑1 (MQ=255)
tGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt < 1:2522871/68‑1 (MQ=255)
tGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt < 1:983109/68‑1 (MQ=255)
tGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt < 1:2495094/68‑1 (MQ=255)
tGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt < 1:2480885/68‑1 (MQ=255)
tGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt < 1:2476848/68‑1 (MQ=255)
tGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt < 1:3260066/68‑1 (MQ=255)
tGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt < 1:454356/68‑1 (MQ=255)
tGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt < 1:630933/68‑1 (MQ=255)
tGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt < 1:876089/68‑1 (MQ=255)
cccGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCt > 1:1027170/1‑69 (MQ=255)
cccGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCt > 1:3886/1‑69 (MQ=255)
cccGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCt > 1:405690/1‑69 (MQ=255)
cccGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCt > 1:2453729/1‑69 (MQ=255)
cccGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCt > 1:1257236/1‑69 (MQ=255)
cccGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCt > 1:567365/1‑69 (MQ=255)
ttACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTaa > 1:3245279/1‑68 (MQ=255)
ttACTCAACAA‑CAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAAcc > 1:755618/1‑69 (MQ=255)
aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:1088274/1‑45 (MQ=255)
aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:480839/1‑45 (MQ=255)
aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:1323877/1‑45 (MQ=255)
aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:778526/1‑45 (MQ=255)
aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:1949803/1‑45 (MQ=255)
aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:3219782/1‑45 (MQ=255)
aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:1524693/1‑45 (MQ=255)
aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:283708/1‑45 (MQ=255)
aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:2788436/1‑45 (MQ=255)
aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:2681150/1‑45 (MQ=255)
aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:2153167/1‑45 (MQ=255)
aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:2513326/1‑45 (MQ=255)
aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:1820374/1‑45 (MQ=255)
aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:1802764/1‑45 (MQ=255)
aacaacCAGGCCAGCGCAACTTCTGCTCGCGTTGCATCAAGttct > 1:1945240/1‑45 (MQ=255)
acCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGa < 1:1659871/69‑1 (MQ=255)
acCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGa < 1:1268716/69‑1 (MQ=255)
gcAACTTTTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:1084224/69‑1 (MQ=255)
gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:1533356/69‑1 (MQ=255)
gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:3059982/69‑1 (MQ=255)
gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:902357/69‑1 (MQ=255)
gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:2018486/69‑1 (MQ=255)
gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:2061074/69‑1 (MQ=255)
gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:1155640/69‑1 (MQ=255)
gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:413182/69‑1 (MQ=255)
gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:3295611/69‑1 (MQ=255)
gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:174085/69‑1 (MQ=255)
gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:3231949/69‑1 (MQ=255)
gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:2484990/69‑1 (MQ=255)
gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:3074650/69‑1 (MQ=255)
cAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:2915906/68‑1 (MQ=255)
cAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:1849874/68‑1 (MQ=255)
cAACTTGTGCTCGTGACGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:1787698/68‑1 (MQ=255)
cTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:2219348/65‑1 (MQ=255)
cGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGct < 1:687059/68‑1 (MQ=255)
tCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGctttctt > 1:2223937/1‑69 (MQ=255)
tCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGctttctt > 1:1535190/1‑69 (MQ=255)
tCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGctttctt > 1:3157674/1‑69 (MQ=255)
tCGCCCACAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGctttctt > 1:1338/1‑69 (MQ=255)
|
ATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTT > minE/265754‑265883
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
