Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
297,993 |
C→T |
25.1% |
R218R (CGC→CGT) |
mdlA → |
fused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 297,993 | 0 | C | T | 25.1%
| 118.6
/ 32.6
| 95 | R218R (CGC→CGT) | mdlA | fused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (34/37); new base T (6/18); total (40/55) |
| Fisher's exact test for biased strand distribution p-value = 5.86e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 |
Only 100 of 105 total aligned reads displayed.
TTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATT > minE/297926‑298059
|
ttAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGcc > 1:276379/1‑69 (MQ=255)
ttAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGcc > 1:3408667/1‑69 (MQ=255)
ttAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGcc > 1:781479/1‑69 (MQ=255)
ttAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGcc > 1:1745607/1‑69 (MQ=255)
ttAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGcc > 1:2280452/1‑69 (MQ=255)
ttAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGcc > 1:386098/1‑69 (MQ=255)
ttAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGcc > 1:1342381/1‑69 (MQ=255)
ttAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGcc > 1:1235517/1‑69 (MQ=255)
ttAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGcc > 1:94224/1‑69 (MQ=255)
ttAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGcc > 1:179337/1‑69 (MQ=255)
aCCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTa < 1:805272/69‑1 (MQ=255)
aCCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTa < 1:2997616/69‑1 (MQ=255)
aCCCAGGAAAGCCTCACCAGTATCCCCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTa < 1:2148457/69‑1 (MQ=255)
aaGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTa < 1:2100577/61‑1 (MQ=255)
cTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGAt > 1:904510/1‑69 (MQ=255)
cAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCg < 1:169836/68‑1 (MQ=255)
cAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCg < 1:637401/68‑1 (MQ=255)
aGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGaa < 1:1092768/69‑1 (MQ=255)
aGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGaa < 1:1973107/69‑1 (MQ=255)
aGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGaa < 1:3361594/69‑1 (MQ=255)
aGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGaa < 1:3122681/69‑1 (MQ=255)
aGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGaa < 1:3056483/69‑1 (MQ=255)
aGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGaa < 1:300801/69‑1 (MQ=255)
aGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGaa < 1:1455714/69‑1 (MQ=255)
gTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGaa < 1:877356/68‑1 (MQ=255)
gTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGaa < 1:949411/68‑1 (MQ=255)
gTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGaa < 1:1791655/68‑1 (MQ=255)
tCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCg > 1:170115/1‑41 (MQ=255)
tCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGAt > 1:44465/1‑68 (MQ=255)
tCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATa > 1:2213562/1‑69 (MQ=255)
tCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATa > 1:1148542/1‑69 (MQ=255)
tCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATa > 1:1190877/1‑69 (MQ=255)
tCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATa > 1:2816422/1‑69 (MQ=255)
tCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATa > 1:2787862/1‑69 (MQ=255)
tCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCAGCGGATGCCGAAGATa > 1:3389870/1‑69 (MQ=255)
cGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATAcc > 1:2598390/1‑69 (MQ=255)
cGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATAcc > 1:262313/1‑69 (MQ=255)
cGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATAcc > 1:23134/1‑69 (MQ=255)
cGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATAcc > 1:531967/1‑69 (MQ=255)
cGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATAcc > 1:2559345/1‑69 (MQ=255)
cGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATAcc > 1:527625/1‑69 (MQ=255)
cGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATAc > 1:872564/1‑68 (MQ=255)
cGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATAc > 1:2776505/1‑68 (MQ=255)
tGATCCAAGCCTTTGGTCTGCAAGATCGTCAGTCg < 1:3110289/35‑1 (MQ=25)
tGATCAAAGCCTTTGGTCTGGAAGATCGTCAGTCg < 1:110367/35‑1 (MQ=255)
tGATCAAAGCCTTTGGTCTGGAAGATCGTCAGTCg < 1:3049242/35‑1 (MQ=255)
tGATCAAAGCCTTTGGTCTGGAAGATCGTCAGTCg < 1:3353977/35‑1 (MQ=255)
tGATCAAAGCCTTTGGTCTGGAAGATCGTCAGTCg < 1:799371/35‑1 (MQ=255)
tGATCAAAGCCTTTGGTCTGGAAGATCGTCAGTCg < 1:679293/35‑1 (MQ=255)
tGATCAAAGCCTTTGGTCTGGAAGATCGTCAGTCg < 1:35128/35‑1 (MQ=255)
tGATCAAAGCCTTTGGTCTGGAAGATCGTCAGTCg < 1:587852/35‑1 (MQ=255)
tGATCAAAGCCTTTGGTCTGGAAGATCGTCAGTCg < 1:450800/35‑1 (MQ=255)
tGATCAAAGCCTTTGGTCTGGAAGATCGTCAGTCg < 1:492382/35‑1 (MQ=255)
tGATCAAAGCCTTTGGTCTGGAAGATCGTCAGTCg < 1:2661180/35‑1 (MQ=255)
tGATCAAAGCCTTTGGTCTGGAAGATCGTCAGTCg < 1:1239603/35‑1 (MQ=255)
tGATCAAAGCCTTTGGTCTGGAAGATCGTCAGTCg < 1:2648400/35‑1 (MQ=255)
tGATCAAAGCCTTTGGTCTGGAAGATCGTCAGTCg < 1:1347705/35‑1 (MQ=255)
tGATCAAAGCCTTTGGTCTGGAAGATCGTCAGTCg < 1:1478015/35‑1 (MQ=255)
tGATCAAAGCCTTTGGTCTGGAAGATCGTCAGTCg < 1:258941/35‑1 (MQ=255)
tGATCAAAGCCTTTGGTCTGGAAGATCGTCAGTCg < 1:1800745/35‑1 (MQ=255)
tGATCAAAGCCTTTGGTCTGGAAGATCGTCAGTCg < 1:1967956/35‑1 (MQ=255)
tGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGc > 1:1104072/1‑68 (MQ=255)
tGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGc > 1:896219/1‑68 (MQ=255)
tGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGc > 1:2608636/1‑68 (MQ=255)
aTCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCaa > 1:1837457/1‑68 (MQ=255)
aTCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCaa > 1:2287559/1‑68 (MQ=255)
cAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCaaaa > 1:490562/1‑68 (MQ=255)
aGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCaaaaaaaa < 1:2399902/69‑1 (MQ=255)
gTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCg < 1:3136715/67‑1 (MQ=255)
gTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCg < 1:1660193/67‑1 (MQ=255)
gTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCg < 1:2372564/67‑1 (MQ=255)
gTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCg < 1:2409874/67‑1 (MQ=255)
gTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGAAACCGGCAAAAAAAACATGCg < 1:528798/67‑1 (MQ=255)
gAAGATCGTCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAAGATGCGGGTGgcg > 1:886200/1‑69 (MQ=255)
gAAGATCGTCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGgcg > 1:2839271/1‑69 (MQ=255)
gAAGATCGTCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGgcg > 1:551528/1‑69 (MQ=255)
gAAGATCGTCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGgcg > 1:1570798/1‑69 (MQ=255)
gAAGATCGTCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGgcg > 1:1629570/1‑69 (MQ=255)
gAAGATAGTCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGgc > 1:90081/1‑68 (MQ=255)
aTCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTa < 1:2258683/69‑1 (MQ=255)
cGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACCTGCGGGTGGCGCGTAtt < 1:2030643/69‑1 (MQ=255)
cGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGt > 1:1798327/1‑59 (MQ=255)
cGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTAtt < 1:2635755/69‑1 (MQ=255)
cGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTAtt < 1:1207620/69‑1 (MQ=255)
cGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTAtt < 1:1348196/69‑1 (MQ=255)
cGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTAtt > 1:2672521/1‑69 (MQ=255)
cGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTAtt < 1:2657098/69‑1 (MQ=255)
cGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTAtt > 1:171790/1‑69 (MQ=255)
cGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTAtt > 1:1795053/1‑69 (MQ=255)
cGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTAtt > 1:2436992/1‑69 (MQ=255)
cGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTAtt > 1:1927836/1‑69 (MQ=255)
cGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTAtt < 1:385130/69‑1 (MQ=255)
cGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTAtt < 1:2120507/69‑1 (MQ=255)
cGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTAtt > 1:2269598/1‑69 (MQ=255)
cGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTAtt > 1:3236966/1‑69 (MQ=255)
cGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTAtt < 1:3122763/69‑1 (MQ=255)
cGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTAtt < 1:237806/69‑1 (MQ=255)
gCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTAtt < 1:598842/68‑1 (MQ=255)
gCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTAtt < 1:585355/68‑1 (MQ=255)
gCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTAtt < 1:1983550/68‑1 (MQ=255)
|
TTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATT > minE/297926‑298059
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
