Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
327,825 |
A→G |
38.7% |
R206R (CGT→CGC) |
aes ← |
acetyl esterase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 327,825 | 0 | A | G | 38.7%
| 26.0
/ 40.7
| 62 | R206R (CGT→CGC) | aes | acetyl esterase |
| Reads supporting (aligned to +/- strand): ref base A (24/14); new base G (9/15); total (33/29) |
| Fisher's exact test for biased strand distribution p-value = 6.83e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 |
TGCCTCTTCGTACATCTGCAAATCCTGTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAAC > minE/327762‑327872
|
tGCCTCTTCGTACATCTGCAAATCCTGTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGACGAGt > 1:238689/1‑69 (MQ=255)
tCTGCAAATCCTGTTGCGTTAAGCCATCCCAGACACCTCCCAACAGACGGCGAGTCACGGAAt > 1:2244661/1‑63 (MQ=255)
tCTGCAAATCCTGTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGt > 1:3306770/1‑68 (MQ=255)
tCTGCAAATCCTGTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTa > 1:227057/1‑69 (MQ=255)
tCTGCAAATCCTGTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTa > 1:2957301/1‑69 (MQ=255)
tCTGCAAATCCTGTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTa > 1:1310076/1‑69 (MQ=255)
cTGCAAATCCTGTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGt < 1:2323698/67‑1 (MQ=255)
tGCAAATCCTGTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTaa > 1:2825954/1‑68 (MQ=255)
tGCAAATCCTGTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTAAt > 1:647537/1‑69 (MQ=255)
cAAATCCTGTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTAATc > 1:2119298/1‑68 (MQ=255)
cAAATCCTGTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTAATc > 1:1975900/1‑68 (MQ=255)
gTTGCGTTAAGCCATCCCAGACACCGCCCAACAGAGGACGAGTCACGGAATCCCGTAATCCGTAAAGcc < 1:2020727/69‑1 (MQ=255)
gTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTAATCCGTAAAGcc < 1:1763987/69‑1 (MQ=255)
gTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCTTAAAGcc < 1:2966965/69‑1 (MQ=255)
gTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGcc < 1:1016632/69‑1 (MQ=255)
gTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGcc < 1:1726121/69‑1 (MQ=255)
gTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGcc < 1:2250901/69‑1 (MQ=255)
gTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGcc < 1:798670/69‑1 (MQ=255)
gTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGcc < 1:814792/69‑1 (MQ=255)
gTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGcc < 1:817862/69‑1 (MQ=255)
gTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGcc < 1:960979/69‑1 (MQ=255)
gCGTTAAGCCATCCCAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCa < 1:490263/68‑1 (MQ=255)
gCGTTAAGCCATCCCAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCa < 1:543592/68‑1 (MQ=255)
gCGTTAAGCCATCCCAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCa < 1:1306863/68‑1 (MQ=255)
aTCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCaa < 1:3167626/69‑1 (MQ=255)
aTCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCaa < 1:3188300/69‑1 (MQ=255)
aTCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCaa < 1:2899617/69‑1 (MQ=255)
aTCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCaa < 1:610286/69‑1 (MQ=255)
aTCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCaa < 1:750203/69‑1 (MQ=255)
aTCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCaa < 1:2770935/69‑1 (MQ=255)
aTCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCaa < 1:757101/69‑1 (MQ=255)
aTCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCaa < 1:2371907/69‑1 (MQ=255)
aTCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCaa < 1:1220697/69‑1 (MQ=255)
aTCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCaa < 1:2119672/69‑1 (MQ=255)
aTCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCaa < 1:1999181/69‑1 (MQ=255)
aTCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCaa < 1:1301139/69‑1 (MQ=255)
tCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCaa < 1:797432/68‑1 (MQ=255)
tCCCAGACACCGCCCAACAGACGGCGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCaa < 1:581721/68‑1 (MQ=255)
ccAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCaaaa > 1:923447/1‑68 (MQ=255)
ccAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAAc > 1:1427511/1‑69 (MQ=255)
ccAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAAc > 1:693220/1‑69 (MQ=255)
ccAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAAc > 1:1269027/1‑69 (MQ=255)
ccAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAAc > 1:1189830/1‑69 (MQ=255)
ccAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAAc > 1:1168095/1‑69 (MQ=255)
ccAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAAc > 1:447197/1‑69 (MQ=255)
ccAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAAc > 1:1454683/1‑69 (MQ=255)
ccAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAAc > 1:862056/1‑69 (MQ=255)
ccAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAAc > 1:1619077/1‑69 (MQ=255)
ccAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAAc > 1:2944622/1‑69 (MQ=255)
ccAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAAc > 1:2240148/1‑69 (MQ=255)
ccAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAAc > 1:2625196/1‑69 (MQ=255)
ccAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAAc > 1:927735/1‑69 (MQ=255)
acAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAAc > 1:644387/2‑69 (MQ=255)
gCCCAACAGACGACGAGTCACGGAATCCCGTAATc > 1:2547935/1‑35 (MQ=255)
gCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTaaa > 1:1380480/1‑41 (MQ=255)
gCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCa > 1:1266852/1‑46 (MQ=255)
gCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCa > 1:1572908/1‑46 (MQ=255)
gCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCAt > 1:1415277/1‑47 (MQ=255)
gCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCAt > 1:1056618/1‑47 (MQ=255)
gCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCAt > 1:1461084/1‑47 (MQ=255)
gCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCAt > 1:912104/1‑47 (MQ=255)
cAGACGACGAGTCACGGAATCCCGTAATCCGTATAGcc < 1:1026343/38‑1 (MQ=255)
gacgaGTCACGGAATCCCGTAATCCGTAAAGCCCa < 1:2367591/35‑1 (MQ=255)
|
TGCCTCTTCGTACATCTGCAAATCCTGTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAAC > minE/327762‑327872
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
