Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
388,228 |
C→T |
35.9% |
A49A (GCG→GCA) |
ybdH ← |
predicted oxidoreductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 388,228 | 0 | C | T | 35.9%
| 58.1
/ 49.8
| 86 | A49A (GCG→GCA) | ybdH | predicted oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (28/27); new base T (11/20); total (39/47) |
| Fisher's exact test for biased strand distribution p-value = 1.84e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAA > minE/388167‑388291
|
gACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCgca > 1:1928372/1‑68 (MQ=255)
gACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCgca > 1:3185159/1‑68 (MQ=255)
aCCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCgcagc > 1:2741851/1‑69 (MQ=255)
aCCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCgcagc < 1:163722/69‑1 (MQ=255)
aCCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCgcagc > 1:521918/1‑69 (MQ=255)
aCCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCgcagc > 1:525870/1‑69 (MQ=255)
aCCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCgcagc > 1:2136198/1‑69 (MQ=255)
aCCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCgcagc < 1:3038406/69‑1 (MQ=255)
aCCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCgcagc > 1:2486192/1‑69 (MQ=255)
aCCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCgcagc > 1:2520576/1‑69 (MQ=255)
aCCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCgcagc > 1:2640010/1‑69 (MQ=255)
aCCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCgcagc < 1:1226774/69‑1 (MQ=255)
aCCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCgcagc > 1:85434/1‑69 (MQ=255)
aCCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCgcagc > 1:897042/1‑69 (MQ=255)
aCCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCgcagc > 1:270759/1‑69 (MQ=255)
aCCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCgcagc > 1:1091459/1‑69 (MQ=255)
aCCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCgcagc > 1:10509/1‑69 (MQ=255)
tGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGgcgc > 1:2472442/1‑61 (MQ=255)
tGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGcgcg < 1:81345/62‑1 (MQ=255)
tGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGcgcg < 1:2139727/62‑1 (MQ=255)
tGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGcgcg > 1:156362/1‑62 (MQ=255)
tGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGcgcg < 1:491254/62‑1 (MQ=255)
tGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGcgcg < 1:484020/62‑1 (MQ=255)
tGCTTTGCCCCTGGAAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGcgcg > 1:486383/1‑62 (MQ=255)
tCCAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGcgcg < 1:778118/45‑1 (MQ=255)
tcAAACGCAGGCGGCAGTTTGGTTTGTGCCGCAGCAATGGCGCTTTCGCCGTAGATCCACACCGCGCga < 1:1812832/68‑1 (MQ=255)
tcAAACGCAGGCGGCAGTTTGGTTTGTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCga < 1:766766/68‑1 (MQ=255)
tcAAACGCAGGCGGCAGTTTGGTTTGTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCga < 1:1099262/68‑1 (MQ=255)
tcAAACGCAGGCGGCAGTTTGGTTTGTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCga < 1:125156/68‑1 (MQ=255)
tcAAACGCAGGCGGCAGTTTGGTTTGTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCga < 1:2565690/68‑1 (MQ=255)
tcAAACGCAGGCGGCAGTTTGGTTTGTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCga < 1:828505/68‑1 (MQ=255)
tcAAACGCAGGCGGCAGTTTGGTTTGTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCga < 1:1280377/68‑1 (MQ=255)
tcAAACGCAGGCGGCAGTTTGGTTTGTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCga < 1:2924843/68‑1 (MQ=255)
tcAAACGCAGGCGGCAGTTTGGTTTGTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCga < 1:2975295/68‑1 (MQ=255)
tcAAACGCAGGCGGCAGTTTGGTTTGTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCga < 1:3015583/68‑1 (MQ=255)
tcAAACGCAGGCGGCAGTTTGGTTTGTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCga < 1:304726/68‑1 (MQ=255)
tcAAACGCAGGCGGCAGTTTGGTTTGTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCga < 1:316721/68‑1 (MQ=255)
tcAAACGCAGGCGGCAGTTTGGTTTGTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCga < 1:2152685/68‑1 (MQ=255)
tcAAACGCAGGCGGCAGTTTGGTTTGTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCga < 1:691657/68‑1 (MQ=255)
tcAAACGCAGGCGGCAGTTTGGTTTGTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCga < 1:2114552/68‑1 (MQ=255)
tcAAACGCAGGCGGCAGTTTGGTTTGTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCga < 1:1786186/68‑1 (MQ=255)
tcAAACGCAGGCGGCAGTTTGGTTTGTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCga < 1:344117/68‑1 (MQ=255)
tcAAACGCAGGCGGCAGTTTGGTTTGTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCga < 1:369670/68‑1 (MQ=255)
ccAACCGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACcgcgcg > 1:1020956/1‑68 (MQ=255)
ccAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACcgcgcg > 1:985242/1‑68 (MQ=255)
ccAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACcgcgcg > 1:617181/1‑68 (MQ=255)
ccAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACcgcgcg > 1:1927049/1‑68 (MQ=255)
ccAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACcgcgcg > 1:1691867/1‑68 (MQ=255)
cAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCga > 1:2422431/1‑68 (MQ=255)
cAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCga > 1:295100/1‑68 (MQ=255)
cAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCga > 1:1105902/1‑68 (MQ=255)
cAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCga > 1:894977/1‑68 (MQ=255)
cAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCga > 1:3250395/1‑68 (MQ=255)
cAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCga > 1:1447303/1‑68 (MQ=255)
cAAACGCAGGCGGCAGTTTGGTTTGTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCga < 1:2215046/68‑1 (MQ=255)
cGCAGGCGGCAGTTTGGTTTGTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCga < 1:139316/64‑1 (MQ=255)
cTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGcgcg < 1:3435776/37‑1 (MQ=255)
tGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAgttg < 1:1955220/69‑1 (MQ=255)
tGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAgttg < 1:284573/69‑1 (MQ=255)
tGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAgttg < 1:53470/69‑1 (MQ=255)
tGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAgttg < 1:70221/69‑1 (MQ=255)
tGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAgttg < 1:1292113/69‑1 (MQ=255)
tGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAgttg < 1:859324/69‑1 (MQ=255)
tGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAgttg < 1:1039591/69‑1 (MQ=255)
tGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAgttg < 1:2035608/69‑1 (MQ=255)
tGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAgttg < 1:2235212/69‑1 (MQ=255)
tGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAgttg < 1:433340/69‑1 (MQ=255)
tGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAgttg < 1:3248426/69‑1 (MQ=255)
tGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAgttg < 1:3350934/69‑1 (MQ=255)
aaGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACAC‑‑CGCGAGAAAGTTGTTCATCAg < 1:2320482/69‑1 (MQ=255)
gtttggtttgCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAg < 1:2086838/69‑1 (MQ=255)
gtttggtttgCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAg < 1:1133288/69‑1 (MQ=255)
gtttggtttgCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAg < 1:1484959/69‑1 (MQ=255)
gtttggtttgCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAg < 1:1623670/69‑1 (MQ=255)
gtttggtttgCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAg < 1:485786/69‑1 (MQ=255)
ttgTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGaaa > 1:3287685/1‑67 (MQ=255)
ttgTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGaaa > 1:1436084/1‑67 (MQ=255)
ttgTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGaaa > 1:2054610/1‑67 (MQ=255)
ttgTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGaaa > 1:1270570/1‑67 (MQ=255)
ttgTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGaaa > 1:367740/1‑67 (MQ=255)
ttgTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGaaa > 1:2253766/1‑67 (MQ=255)
ttgTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGaaa > 1:872369/1‑67 (MQ=255)
ttgTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGaaa > 1:2336932/1‑67 (MQ=255)
ttgTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGaaa > 1:909928/1‑67 (MQ=255)
ttgTGCCGCAGCAATGGCGCGTTCGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGaaa > 1:2709382/1‑67 (MQ=255)
ttgTGCCGCAGCAATGGCGCGTTCGCCGTAGATCAACACCGCGCGAGAAAGTTGTTCATCAGTGaaa > 1:130927/1‑67 (MQ=255)
|
GACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAA > minE/388167‑388291
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
