Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
389,226 |
T→C |
45.3% |
C248C (TGT→TGC) |
ybdL → |
methionine aminotransferase, PLP‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 389,226 | 0 | T | C | 45.3%
| 12.0
/ 71.0
| 86 | C248C (TGT→TGC) | ybdL | methionine aminotransferase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (25/22); new base C (9/30); total (34/52) |
| Fisher's exact test for biased strand distribution p-value = 7.44e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01 |
AGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAAT > minE/389159‑389292
|
aGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATtgtg > 1:2753837/1‑69 (MQ=255)
ggCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTgc < 1:1114604/69‑1 (MQ=255)
ggCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTgc < 1:3221641/69‑1 (MQ=255)
gCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTgc < 1:3430252/68‑1 (MQ=255)
gCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAgcg > 1:1339321/1‑69 (MQ=255)
ggTGTCATCGTTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTtgcgcctgcgcc > 1:3142824/1‑69 (MQ=255)
ggTGTCATCGTTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTtgcgcctgcgcc > 1:3303420/1‑69 (MQ=255)
ggTGTCATCGTTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTtgcgcctgcgcc > 1:799519/1‑69 (MQ=255)
ggTGTCATCGTTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTtgcgcctgcgcc > 1:1982728/1‑69 (MQ=255)
ggTGTCATCGTTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTtgcgcctgcgcc > 1:2809399/1‑69 (MQ=255)
ggTGTCATCGTTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTtgcgcctgcgcc > 1:1683570/1‑69 (MQ=255)
tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:48634/1‑68 (MQ=255)
tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:1491399/1‑68 (MQ=255)
tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:3259853/1‑68 (MQ=255)
tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:3388225/1‑68 (MQ=255)
tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:2011179/1‑68 (MQ=255)
tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:971379/1‑68 (MQ=255)
tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:97426/1‑68 (MQ=255)
tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:210468/1‑68 (MQ=255)
ttcttcATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:1386122/1‑67 (MQ=255)
ttcttcATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:282809/1‑67 (MQ=255)
ttcttcATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:1470549/1‑67 (MQ=255)
ttcttcATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:516525/1‑67 (MQ=255)
ttcttcATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:2637388/1‑67 (MQ=255)
ttcttcATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:2547129/1‑67 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2512579/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2660547/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2826495/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2520722/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:284910/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:1070505/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2880696/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:289473/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2954733/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2994785/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:3063319/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:3402217/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:795639/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:827283/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:956851/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:988601/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:1053493/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2006572/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2420899/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:1375073/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2224989/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2238681/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:1331200/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:1246128/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2319101/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:1118673/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2398030/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2400174/68‑2 (MQ=255)
gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:1082285/68‑2 (MQ=255)
cAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:1198923/67‑2 (MQ=255)
tCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAgg > 1:687168/1‑68 (MQ=255)
atatGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTa > 1:2093868/1‑68 (MQ=255)
atatGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTAc > 1:3281154/1‑69 (MQ=255)
atatGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTAc > 1:2185521/1‑69 (MQ=255)
atatGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTAc > 1:2280349/1‑69 (MQ=255)
tGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATc > 1:1456229/1‑69 (MQ=255)
gACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGAGATCCGCAAAGTGCATc > 1:1828158/1‑68 (MQ=255)
gACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGAGATCCGCAAAGTGCATc > 1:1642729/1‑68 (MQ=255)
gACAGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCG‑CCATCAGCGCCGAGATCCGCAAAGTGCATCAg > 1:490343/1‑69 (MQ=255)
cGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTa < 1:2461463/69‑1 (MQ=255)
cGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTa < 1:2611535/69‑1 (MQ=255)
cGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTa < 1:1192918/69‑1 (MQ=255)
cGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTa < 1:122172/69‑1 (MQ=255)
cGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTa < 1:777118/69‑1 (MQ=255)
cGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTa < 1:1307378/69‑1 (MQ=255)
cGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTa < 1:3372185/69‑1 (MQ=255)
ggCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTa < 1:1420467/68‑1 (MQ=255)
tGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCtttt > 1:1386311/1‑69 (MQ=255)
tGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCtttt > 1:2311586/1‑69 (MQ=255)
ggTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTc > 1:987397/1‑40 (MQ=255)
ggTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCg > 1:971663/1‑69 (MQ=255)
tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:2041407/69‑1 (MQ=255)
tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:1285544/69‑1 (MQ=255)
tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:527047/69‑1 (MQ=255)
tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:92415/69‑1 (MQ=255)
tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:3360409/69‑1 (MQ=255)
tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:783210/69‑1 (MQ=255)
tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:2623186/69‑1 (MQ=255)
tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:1624180/69‑1 (MQ=255)
tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:3125334/69‑1 (MQ=255)
tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:914414/69‑1 (MQ=255)
tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTAGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:1177302/69‑1 (MQ=255)
tgtgTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGaa < 1:2403468/68‑1 (MQ=255)
tgtgTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGaa < 1:2846773/68‑1 (MQ=255)
tgtgTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGaa < 1:2343094/68‑1 (MQ=255)
tgtgTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGaa < 1:2847338/68‑1 (MQ=255)
tgtgTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAAt > 1:841760/1‑69 (MQ=255)
tgtgTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAAt > 1:3148235/1‑69 (MQ=255)
tgtgTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAAt > 1:706978/1‑69 (MQ=255)
|
AGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAAT > minE/389159‑389292
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
