Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
516,305 |
T→C |
100% |
T169T (ACT→ACC) |
bioB → |
biotin synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 516,305 | 0 | T | C | 81.7%
| 109.8
/ 13.6
| 71 | T169T (ACT→ACC) | bioB | biotin synthase |
| Reads supporting (aligned to +/- strand): ref base T (12/1); new base C (57/0); total (70/1) |
| Fisher's exact test for biased strand distribution p-value = 1.86e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CAACCTGGACACCTCGCCGGAGTTTTACGGCAATATCATCACCACACGCACTTATCAGGAACGCCTCGATACGCTGGAAAAAGTGCGCGATGCCGGGATCAAAGTCTGTTCTGG > minE/516254‑516367
|
caaccTGGACACCTCGCCGGAGTTTTACGGCAATATCATCACCACACGCACTTATCAGGAACGCCTCGa < 1:2792318/69‑1 (MQ=255)
cGCCGGAGTTTTACGGCAATATCATCACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGaaaaa > 1:986053/1‑68 (MQ=255)
cGCCGGAGTTTTACGGCAATATCATCACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGaaaaa > 1:3303099/1‑68 (MQ=255)
cGCCGGAGTTTTACGGCAATATCATCACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGaaaaa > 1:3358487/1‑68 (MQ=255)
cGCCGGAGTTTTACGGCAATATCATCACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAg > 1:1291676/1‑69 (MQ=255)
cGCCGGAGTTTTACGGCAATATCATCACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAg > 1:49690/1‑69 (MQ=255)
cGCCGGAGTTTTACGGCAATATCATCACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAg > 1:525544/1‑69 (MQ=255)
cGCCGGAGTTTTACGGCAATATCATCACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAg > 1:2123235/1‑69 (MQ=255)
cGCCGGAGTTTTACGGCAATATCATCACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAg > 1:2522401/1‑69 (MQ=255)
cGCCGGAGTTTTACGGCAATATCATCACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAg > 1:2618858/1‑69 (MQ=255)
cGCCGGAGTTTTACGGCAATATCATCACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAg > 1:2841410/1‑69 (MQ=255)
ttACGGCAATATCATCACCACACGCACTTATCAGGAACGCCTCGATACGCTGGAAAAAGTGCGCGATGc > 1:1574158/1‑69 (MQ=255)
ttACGGCAATATCATCACCACACGCACTTATCAGGAACGCCTCGATACGCTGGAAAAAGTGCGCGATGc > 1:2981990/1‑69 (MQ=255)
ttACGGCAATATCATCACCACACGCACTTATCAGGAACGCCTCGATACGCTGGAAAAAGTGCGCGATGc > 1:3091323/1‑69 (MQ=255)
ttACGGCAATATCATCACCACACGCACTTATCAGGAACGCCTCGATACGCTGGAAAAAGTGCGCGATGc > 1:3118471/1‑69 (MQ=255)
ttACGGCAATATCATCACCACACGCACTTATCAGGAACGCCTCGATACGCTGGAAAAAGTGCGCGATGc > 1:2116112/1‑69 (MQ=255)
gCAATATCATCACCACACGCACTTATCAGGAACGCCTCGATACGCTGGAAAAAGTGCGCGATGCCggg > 1:1026760/1‑68 (MQ=255)
gCAATATCATCACCACACGCACTTATCAGGAACGCCTCGATACGCTGGAAAAAGTGCGCGATGCCggg > 1:2725367/1‑68 (MQ=255)
gCAATATCATCACCACACGCACTTATCAGGAACGCCTCGATACGCTGGAAAAAGTGCGCGATGCCggg > 1:1859776/1‑68 (MQ=255)
atcatcACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCgaga > 1:809136/1‑56 (MQ=255)
atcatcACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTaaa > 1:952745/1‑69 (MQ=255)
atcatcACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTaaa > 1:118431/1‑69 (MQ=255)
atcatcACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTaaa > 1:2502942/1‑69 (MQ=255)
atcatcACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTaaa > 1:2873515/1‑69 (MQ=255)
atcatcACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTaaa > 1:1311774/1‑69 (MQ=255)
atcatcACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTaaa > 1:1290234/1‑69 (MQ=255)
atcatcACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTaaa > 1:867352/1‑69 (MQ=255)
atcatcACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTaaa > 1:158393/1‑69 (MQ=255)
atcatcACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTaaa > 1:728250/1‑69 (MQ=255)
atcatcACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTaaa > 1:186274/1‑69 (MQ=255)
atcatcACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTaaa > 1:1829198/1‑69 (MQ=255)
atcatcACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTaaa > 1:179740/1‑69 (MQ=255)
atcatcACCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTaa > 1:220578/1‑68 (MQ=255)
atcatcACCACACGCACTTATCAGGAACGCCTCGATACGCTGGAAAAAGTGCGCGATGCCGGGATCaaa > 1:771058/1‑69 (MQ=255)
atcatcACCACACGCACTTATCAGGAACGCCTCGATACGCTGGAAAAAGTGCGCGATGCCGGGATCaaa > 1:2316898/1‑69 (MQ=255)
catcaCCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGctg > 1:335652/1‑57 (MQ=255)
catcaCCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAg > 1:924879/1‑68 (MQ=255)
catcaCCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGt > 1:518803/1‑69 (MQ=255)
catcaCCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGt > 1:2911560/1‑69 (MQ=255)
catcaCCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGt > 1:2875191/1‑69 (MQ=255)
catcaCCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGt > 1:3226949/1‑69 (MQ=255)
catcaCCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGt > 1:2559395/1‑69 (MQ=255)
catcaCCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGt > 1:2391428/1‑69 (MQ=255)
catcaCCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGt > 1:1824573/1‑69 (MQ=255)
catcaCCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGt > 1:177456/1‑69 (MQ=255)
catcaCCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGt > 1:2763728/1‑69 (MQ=255)
catcaCCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGt > 1:2284590/1‑69 (MQ=255)
catcaCCACCCGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGt > 1:2410389/1‑69 (MQ=255)
catcaCCACCCGCACCTATCAGGAACGTCATGATACGCTGGAAAAAGTGCGAGAAGCTGGGa > 1:638483/1‑62 (MQ=255)
catcaCCACCCGCACATATCAGGAACGTCTTGATACGCTGGAAAAAGTGAGAGAAGCTGGGAtt > 1:1672651/1‑63 (MQ=255)
caccacACGCACTTATCAGGAACGCCTCGATACGCTGGAAAAAGTGCGCGATGCCGGGATCAAAGTCTg > 1:2541013/1‑69 (MQ=255)
caccacACGCACTTATCAGGAACGCCTCGATACGCTGGAAAAAGTGCGCGATGCCGGGATCAAAGTCTg > 1:1258678/1‑69 (MQ=255)
ccGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGTCTGTTCAgg > 1:835675/2‑69 (MQ=255)
ccGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGTCTGTTCAgg > 1:83141/2‑69 (MQ=255)
ccGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGTCTGTTCAgg > 1:487252/2‑69 (MQ=255)
ccGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGTCTGTTCAgg > 1:1580494/2‑69 (MQ=255)
ccGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGTCTGTTCAgg > 1:1428991/2‑69 (MQ=255)
ccGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGTCTGTTCAgg > 1:729876/2‑69 (MQ=255)
ccGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGTCTGTTCAgg > 1:133067/2‑69 (MQ=255)
ccGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGTCTGTTCAgg > 1:1153245/2‑69 (MQ=255)
ccGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGTCTGTTCAgg > 1:1871857/2‑69 (MQ=255)
ccGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGTCTGTTCAgg > 1:2108761/2‑69 (MQ=255)
ccGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGTCTGTTCAgg > 1:2141110/2‑69 (MQ=255)
ccGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGTCTGTTCAgg > 1:2171489/2‑69 (MQ=255)
ccGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGTCTGTTCAgg > 1:3267887/2‑69 (MQ=255)
ccGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGTCTGTTCAgg > 1:2394844/2‑69 (MQ=255)
ccGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGTCTGTTCAgg > 1:2480938/2‑69 (MQ=255)
ccGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGTCTGTTCAgg > 1:2911204/2‑69 (MQ=255)
ccGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGTCTGTTCAgg > 1:2611706/2‑69 (MQ=255)
ccGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGTCTGTTCAgg > 1:2690297/2‑69 (MQ=255)
ccGCACCTATCAGGAACGTCTTGATACGCTGGAAAAAGTGCGAGAAGCTGGGATTAAAGTCTGTTCAgg > 1:2740157/2‑69 (MQ=255)
|
CAACCTGGACACCTCGCCGGAGTTTTACGGCAATATCATCACCACACGCACTTATCAGGAACGCCTCGATACGCTGGAAAAAGTGCGCGATGCCGGGATCAAAGTCTGTTCTGG > minE/516254‑516367
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
